BMRB: 16851

The structure of E-protein activation domain 1 bound to the KIX domain of CBP/p300 elucidates leukemia induction by E2A-PBX1

Authors

Denis, C.M., Chitayat, S., Plevin, M.J., Liu, S., Spencer, H.L., Ikura, M., LeBrun, D.P., Smith, S.P.

Assembly

KIX ETAD1

Entity

1. KIX (polymer, Thiol state: not present), 87 monomers, 10331.68 Da Detail

GVRKGWHEHV TQDLRSHLVH KLVQAIFPTP DPAALKDRRM ENLVAYAKKV EGDMYESANS RDEYYHLLAE KIYKIQKELE EKRRSRL

2. ETAD1 (polymer, Thiol state: not present), 19 monomers, 2020.176 Da Detail

GSGTDKELSD LLDFSAMFS

Total weight

12351.855 Da

Max. entity weight

10331.68 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 85.4 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.4 % (1084 of 1270)	80.8 % (539 of 667)	88.7 % (438 of 494)	98.2 % (107 of 109)
Backbone	97.0 % (611 of 630)	95.8 % (205 of 214)	97.4 % (305 of 313)	98.1 % (101 of 103)
Sidechain	77.5 % (574 of 741)	73.7 % (334 of 453)	83.0 % (234 of 282)	100.0 % (6 of 6)
Aromatic	43.1 % (44 of 102)	47.1 % (24 of 51)	38.0 % (19 of 50)	100.0 % (1 of 1)
Methyl	89.6 % (95 of 106)	96.2 % (51 of 53)	83.0 % (44 of 53)

1. KIX

GVRKGWHEHV TQDLRSHLVH KLVQAIFPTP DPAALKDRRM ENLVAYAKKV EGDMYESANS RDEYYHLLAE KIYKIQKELE EKRRSRL

2. ETAD1

GSGTDKELSD LLDFSAMFS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	KIX	natural abundance		1.2 mM
2	ETAD1	[U-13C; U-15N]		0.6 mM
3	DSS	natural abundance		1 mM
4	MES	natural abundance		20 mM
5	beta-mercaptoethanol	natural abundance		1 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
6	KIX	[U-13C; U-15N]		0.6 mM
7	ETAD1	natural abundance		1.2 mM
8	DSS	natural abundance		1 mM
9	MES	natural abundance		20 mM
10	beta-mercaptoethanol	natural abundance		1 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KWF, Strand ID: A, B Detail

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Release date

2012-08-01

Citation

Functional redundancy between the transcriptional activation domains of E2A is mediated by binding to the KIX domain of CBP/p300
Denis, C.M., Langelaan, D.N., Kirlin, A.C., Chitayat, S., Munro, K., Spencer, H.L., LeBrun, D.P., Smith, S.P.
Nucleic Acids Res. (2014), 42, 7370-7382, PubMed 24682819 , DOI 10.1093/nar/gku206 , Abstract

Related entities 1. KIX, : 6 : 23 entities Detail

Related entities 2. ETAD1, : 1 : 13 : 11 entities Detail

Interaction partners 1. KIX, : 1 interactors Detail

Interaction partners 2. ETAD1, : 64 interactors Detail

Experiments performed 10 experiments Detail