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BMRB: 16862

2LAN

Chemical shift assignments of CaBP1 with 3 Ca2+ bound

Authors

Ames, J.

Assembly

CaBP1

Entity

1. CaBP1 (polymer, Thiol state: all free), 167 monomers, 19429.80 Da Detail

MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

2. CA (non-polymer), 40.078 Da

Total weight

19469.879 Da

Max. entity weight

19429.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 65.4 %, Completeness (bb): 85.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.4 % (1277 of 1953)	61.5 % (633 of 1030)	67.2 % (503 of 749)	81.0 % (141 of 174)
Backbone	85.7 % (852 of 994)	84.9 % (292 of 344)	86.2 % (420 of 487)	85.9 % (140 of 163)
Sidechain	50.4 % (560 of 1112)	49.7 % (341 of 686)	52.5 % (218 of 415)	9.1 % (1 of 11)
Aromatic	31.9 % (37 of 116)	63.8 % (37 of 58)	0.0 % (0 of 58)
Methyl	81.6 % (124 of 152)	81.6 % (62 of 76)	81.6 % (62 of 76)

1. CaBP1

MGNCVKYPLR NLSRKDRSLR PEEIEELREA FREFDKDKDG YINCRDLGNC MRTMGYMPTE MELIELSQQI NMNLGGHVDF DDFVELMGPK LLAETADMIG VKELRDAFRE FDTNGDGEIS TSELREAMRK LLGHQVGHRD IEEIIRDVDL NGDGRVDFEE FVRMMSR

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CaBP1	[U-98% 13C; U-98% 15N]	1.0 mM
2	calcium	natural abundance	5.0 mM
3	TRIS	natural abundance	10.0 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.47 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2LAN, Strand ID: A Detail

Release date

2010-09-23

Citation

1H, 15N, and 13C chemical shift assignments of calcium-binding protein 1 with Ca2+ bound at EF1, EF3 and EF4
Park, S., Li, C., Ames, J.B.
Biomol. NMR Assign. (2010), 4, 159-161, PubMed 20503119 , DOI 10.1007/s12104-010-9235-8 , Abstract

Related entities 1. CaBP1, : 1 : 1 : 2 : 296 entities Detail

Interaction partners 1. CaBP1, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC