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BMRB: 16872

2KWQ

Mcm10 C-terminal DNA binding domain

Authors

Robertson, P.D., Chagot, B., Chazin, W.J., Eichman, B.F.

Assembly

Mcm10 C-terminal DNA binding domain

Entity

1. Mcm10 (polymer, Thiol state: free and other bound), 92 monomers, 10573.30 Da Detail

GPMGMQSIRE QSCRVVTCKT CKYTHFKPKE TCVSENHDFH WHNGVKRFFK CPCGNRTISL DRLPKKHCST CGLFKWERVG MLKEKTGPKL GG

2. ZN (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

10704.118 Da

Max. entity weight

10573.3 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS18:SG	2:ZN1:ZN
2	na	sing	1:CYS21:SG	2:ZN1:ZN
3	na	sing	1:CYS32:SG	2:ZN1:ZN
4	na	sing	1:HIS37:SG	2:ZN1:ZN
5	na	sing	1:CYS51:SG	2:ZN1:ZN
6	na	sing	1:CYS53:SG	2:ZN1:ZN
7	na	sing	1:CYS68:SG	2:ZN1:ZN
8	na	sing	1:CYS71:SG	2:ZN1:ZN

Source organism

Xenopus laevis

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 85.7 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.7 % (947 of 1105)	96.9 % (568 of 586)	67.3 % (286 of 425)	98.9 % (93 of 94)
Backbone	82.5 % (447 of 542)	96.3 % (181 of 188)	67.4 % (180 of 267)	98.9 % (86 of 87)
Sidechain	90.1 % (582 of 646)	97.2 % (387 of 398)	78.0 % (188 of 241)	100.0 % (7 of 7)
Aromatic	49.0 % (50 of 102)	90.2 % (46 of 51)	4.1 % (2 of 49)	100.0 % (2 of 2)
Methyl	93.5 % (58 of 62)	93.5 % (29 of 31)	93.5 % (29 of 31)

1. Mcm10

GPMGMQSIRE QSCRVVTCKT CKYTHFKPKE TCVSENHDFH WHNGVKRFFK CPCGNRTISL DRLPKKHCST CGLFKWERVG MLKEKTGPKL GG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 15N]	920 uM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	D2O	natural abundance	5 %
5	H2O	natural abundance	95 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	entity_1	[U-100% 15N]	300 uM
7	sodium phosphate	natural abundance	25 mM
8	sodium chloride	natural abundance	100 mM
9	D2O	natural abundance	5 %
10	H2O	natural abundance	95 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
11	entity_1	[U-100% 13C]	920 uM
12	sodium phosphate	natural abundance	25 mM
13	sodium chloride	natural abundance	100 mM
14	D2O	natural abundance	5 %
15	H2O	natural abundance	95 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	entity_1	[U-100% 13C; U-100% 15N]	920 uM
17	sodium phosphate	natural abundance	25 mM
18	sodium chloride	natural abundance	100 mM
19	D2O	natural abundance	5 %
20	H2O	natural abundance	95 %

Sample #5

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
21	entity_1	natural abundance	920 uM
22	sodium phosphate	natural abundance	25 mM
23	sodium chloride	natural abundance	100 mM
24	D2O	natural abundance	5 %
25	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: 2.22 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: 2.22 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KWQ, Strand ID: A Detail

Release date

2010-05-25

Citation

Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif
Robertson, P.D., Chagot, B., Chazin, W.J., Eichman, B.F.
J. Biol. Chem. (2010), 285, 22942-22949, PubMed 20489205 , DOI 10.1074/jbc.M110.131276 , Abstract

Related entities 1. Mcm10, : 1 : 1 : 22 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC