Chemical shift assignments of the 3rd PDZ domain of protein tyrosine phosphatase basophil like (PTP-BL)
TPHVKDYSFV TEDNTFEVKL FKNSSGLGFS FSREDNLIPE QINGSIVRVK KLFPGQPAAE SGKIDVGDVI LKVNGAPLKG LSQQDVISAL RGTAPEVSLL LCRPAPGVLP EIDTPGNSS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.6 % (1022 of 1351) | 75.2 % (529 of 703) | 73.7 % (390 of 529) | 86.6 % (103 of 119) |
Backbone | 89.5 % (621 of 694) | 92.1 % (220 of 239) | 87.3 % (302 of 346) | 90.8 % (99 of 109) |
Sidechain | 65.2 % (499 of 765) | 66.6 % (309 of 464) | 63.9 % (186 of 291) | 40.0 % (4 of 10) |
Aromatic | 16.7 % (12 of 72) | 33.3 % (12 of 36) | 0.0 % (0 of 36) | |
Methyl | 78.2 % (111 of 142) | 81.7 % (58 of 71) | 74.6 % (53 of 71) |
1. PDZ3 PTP-BL
TPHVKDYSFV TEDNTFEVKL FKNSSGLGFS FSREDNLIPE QINGSIVRVK KLFPGQPAAE SGKIDVGDVI LKVNGAPLKG LSQQDVISAL RGTAPEVSLL LCRPAPGVLP EIDTPGNSSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PDZ3 | [U-15N] | 0.5 ~ 1.0 mM | |
2 | TSP | natural abundance | 1.0 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % | |
5 | potassium phosphate | natural abundance | 1.4 mM | |
6 | potassium chloride | natural abundance | 2.7 mM | |
7 | sodium chloride | natural abundance | 137 mM | |
8 | sodium phosphate | natural abundance | 9 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | PDZ3 | [U-100% 13C; U-100% 15N] | 0.5 ~ 1.0 mM | |
10 | TSP | natural abundance | 1.0 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % | |
13 | potassium phosphate | natural abundance | 1.4 mM | |
14 | potassium chloride | natural abundance | 2.7 mM | |
15 | sodium chloride | natural abundance | 137 mM | |
16 | sodium phosphate | natural abundance | 9 mM |