BMRB: 16886

NS2 [27-59]

Authors

Montserret, R., Bartenschlager, R., Penin, F.

Assembly

HCV NS2 [27-59]

Entity

1. HCV NS2 [27-59] (polymer, Thiol state: not present), 33 monomers, 4049.806 Da Detail

KLFLARLIWW LQYFITRAEA HLQVWIPPLN VRG

Formula weight

4049.806 Da

Source organism

Hepacivirus C

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.5 %, Completeness (bb): 79.8 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C
All	85.5 % (348 of 407)	94.8 % (218 of 230)	73.4 % (130 of 177)
Backbone	79.8 % (130 of 163)	98.5 % (64 of 65)	67.3 % (66 of 98)
Sidechain	90.6 % (250 of 276)	93.3 % (154 of 165)	86.5 % (96 of 111)
Aromatic	81.5 % (53 of 65)	82.4 % (28 of 34)	80.6 % (25 of 31)
Methyl	88.5 % (46 of 52)	100.0 % (26 of 26)	76.9 % (20 of 26)

1. HCV NS2 [27-59]

KLFLARLIWW LQYFITRAEA HLQVWIPPLN VRG

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 6, Details mixture of 50% Trifluoro Ethanol D2OH and 50% H2O (v/v).

#	Name	Isotope labeling	Type	Concentration
1	NS2	natural abundance		1.2 mM
2	TFE	[U-2H]		50 % v/v
3	H2O	natural abundance		50 % v/v

Hide sample codition

Protein Blocks Logo

Calculated from 36 models in PDB: 2KWT, Strand ID: A Detail

---

Release date

2011-05-18

Citation

Structural and functional studies of nonstructural protein 2 of the hepatitis C virus reveal its key role as organizer of virion assembly
Jirasko, V., Montserret, R., Lee, J., Gouttenoire, J., Moradpour, D., Penin, F., Bartenschlager, R.
PLoS Pathog. (2010), 6, e1001233-e1001233, PubMed 21187906 , DOI 10.1371/journal.ppat.1001233 , Abstract

Related entities 1. HCV NS2 [27-59], : 1 : 1 : 2 : 28 entities Detail

Interaction partners 1. HCV NS2 [27-59], : 350 interactors Detail

Experiments performed 3 experiments Detail