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BMRB: 16897

2KX2

The solution structure of MTH1821

Authors

Lee, W., Ko, S.

Assembly

MTH1821

Entity

1. MTH1821 (polymer, Thiol state: all free), 96 monomers, 11471.82 Da Detail

MVFYLKVKVE DFGFREDMGL NYVRYRVSGL DEELTEKLIE RLDEDTERDD GDLIITVFYE REYFPFGSEE SKVKMADFIA REEIEMMVFL SSVLED

Formula weight

11471.82 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.4 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.4 % (1017 of 1164)	90.2 % (546 of 605)	81.9 % (379 of 463)	95.8 % (92 of 96)
Backbone	98.1 % (563 of 574)	97.4 % (191 of 196)	98.9 % (280 of 283)	96.8 % (92 of 95)
Sidechain	80.0 % (545 of 681)	86.8 % (355 of 409)	70.1 % (190 of 271)	0.0 % (0 of 1)
Aromatic	20.0 % (24 of 120)	40.0 % (24 of 60)	0.0 % (0 of 60)
Methyl	95.1 % (97 of 102)	96.1 % (49 of 51)	94.1 % (48 of 51)

1. MTH1821

MVFYLKVKVE DFGFREDMGL NYVRYRVSGL DEELTEKLIE RLDEDTERDD GDLIITVFYE REYFPFGSEE SKVKMADFIA REEIEMMVFL SSVLED

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	MTH1821	[U-99% 15N]		1.2 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	HEPES	natural abundance		20 mM
5	NaCl	natural abundance		100 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	MTH1821	[U-100% 13C]		1.2 mM
7	D2O	natural abundance		100 %
8	HEPES	natural abundance		20 mM
9	NaCl	natural abundance		100 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

LACS Plot; CA

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.19 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: -0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KX2, Strand ID: A Detail

Release date

2011-04-07

Citation

Solution structure of MTH1821, a putative structure homologue to RNA polymerase α subunit from Methanobacterium thermoautotrophicum
Ko, S., Kim, H., Yun, J., Yee, A., Arrowsmith, C.H., Cheong, C., Lee, W.
Proteins (2011), 79, 1347-1351, PubMed 21387412 , DOI 10.1002/prot.22956 , Abstract

Related entities 1. MTH1821, : 1 : 2 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	MTH1821	[U-99% 15N]		1.2 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	HEPES	natural abundance		20 mM
5	NaCl	natural abundance		100 mM

2 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

3 3D HNCO

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

4 3D HNCA

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

5 3D HNCACB

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

6 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

7 3D H(CCO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	MTH1821	[U-99% 13C; U-99% 15N]		1.2 mM
11	H2O	natural abundance		90 %
12	D2O	natural abundance		10 %
13	HEPES	natural abundance		20 mM
14	NaCl	natural abundance		100 mM

8 3D HCCH-COSY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	MTH1821	[U-100% 13C]		1.2 mM
7	D2O	natural abundance		100 %
8	HEPES	natural abundance		20 mM
9	NaCl	natural abundance		100 mM

9 3D 1H-15N NOESY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	MTH1821	[U-99% 15N]		1.2 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	HEPES	natural abundance		20 mM
5	NaCl	natural abundance		100 mM

10 3D 1H-13C NOESY

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	MTH1821	[U-100% 13C]		1.2 mM
7	D2O	natural abundance		100 %
8	HEPES	natural abundance		20 mM
9	NaCl	natural abundance		100 mM

nullKeywords methanobacterium thermoautotrophicum, structural genomics