BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.3 % (887 of 972)	91.0 % (465 of 511)	91.3 % (345 of 378)	92.8 % (77 of 83)
Backbone	97.7 % (467 of 478)	98.8 % (163 of 165)	96.6 % (228 of 236)	98.7 % (76 of 77)
Sidechain	87.0 % (494 of 568)	87.3 % (302 of 346)	88.4 % (191 of 216)	16.7 % (1 of 6)
Aromatic	23.3 % (7 of 30)	40.0 % (6 of 15)	0.0 % (0 of 14)	100.0 % (1 of 1)
Methyl	96.2 % (100 of 104)	96.2 % (50 of 52)	96.2 % (50 of 52)

1. UBLCP1

MALPMIVKWG GQEYSVTTLS EDDTVLDLKQ FLKTLTGVLP ERQKLLGLKV KGKPAENDVK LGALKLKPNT KIMMMGTREE S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	UBLI5M	[U-99% 15N]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	HEPES	natural abundance	10 mM
5	NaCl	natural abundance	100 mM
6	DTT	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	UBLI5M	[U-99% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	HEPES	natural abundance	10 mM
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	UBLI5M	[U-100% 13C]	1.5 mM
14	D2O	natural abundance	100 %
15	HEPES	natural abundance	10 mM
16	NaCl	natural abundance	100 mM
17	DTT	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.42 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KX3, Strand ID: A Detail

Release date

2015-06-18

Related entities 1. UBL domain of UBLCP1, I5M, : 1 : 9 : 95 entities Detail

Interaction partners 1. UBL domain of UBLCP1, I5M, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	UBLI5M	[U-99% 15N]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	HEPES	natural abundance	10 mM
5	NaCl	natural abundance	100 mM
6	DTT	natural abundance	5 mM

2 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	UBLI5M	[U-99% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	HEPES	natural abundance	10 mM
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM

3 3D HNCO

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	UBLI5M	[U-99% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	HEPES	natural abundance	10 mM
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM

4 3D HNCA

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	UBLI5M	[U-99% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	HEPES	natural abundance	10 mM
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM

5 3D HNCACB

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	UBLI5M	[U-99% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	HEPES	natural abundance	10 mM
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM

6 3D HBHA(CO)NH

Instrument

Bruker DRX - 500 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	UBLI5M	[U-99% 13C; U-99% 15N]	1.5 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %
10	HEPES	natural abundance	10 mM
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM

7 3D HCCH-TOCSY

Instrument

Bruker DRX - 900 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	UBLI5M	[U-100% 13C]	1.5 mM
14	D2O	natural abundance	100 %
15	HEPES	natural abundance	10 mM
16	NaCl	natural abundance	100 mM
17	DTT	natural abundance	5 mM

8 3D 1H-15N NOESY

Instrument

Bruker DRX - 900 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	UBLI5M	[U-99% 15N]	1.5 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	HEPES	natural abundance	10 mM
5	NaCl	natural abundance	100 mM
6	DTT	natural abundance	5 mM

9 3D 1H-13C NOESY

Instrument

Bruker DRX - 900 MHz equipped with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	UBLI5M	[U-100% 13C]	1.5 mM
14	D2O	natural abundance	100 %
15	HEPES	natural abundance	10 mM
16	NaCl	natural abundance	100 mM
17	DTT	natural abundance	5 mM

nullKeywords UBL domain, UBLCP1, mutant

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Found 1 Document (1.011 seconds)

BMRB: 16899

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. UBL domain of UBLCP1, I5M, : 1 : 9 : 95 entities Detail

Interaction partners 1. UBL domain of UBLCP1, I5M, : 3 interactors Detail

Experiments performed 9 experiments Detail

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