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BMRB: 16912

2M4K

Delta subunit of RNA polymerase from Bacillus subtilis

Authors

Motackova, V., Novacek, J., Zawadska-Kazimierczuk, A., Kazimierczuk, K., Zidek, L., Sanderova, H., Krasny, L., Kozminski, W., Sklenar, V.

Assembly

delta

Entity

1. delta (polymer, Thiol state: not present), 172 monomers, 20267.52 Da Detail

GIKQYSQEEL KEMALVEIAH ELFEEHKKPV PFQELLNEIA SLLGVKKEEL GDRIAQFYTD LNIDGRFLAL SDQTWGLRSW YPYDQLDEET QPTVKAKKKK AKKAVEEDLD LDEFEEIDED DLDLDEVEEE LDLEADDFDE EDLDEDDDDL EIEEDIIDED DEDYDDEEEE IK

Formula weight

20267.52 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.2 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.2 % (1944 of 2021)	95.4 % (1008 of 1057)	96.6 % (758 of 785)	99.4 % (178 of 179)
Backbone	99.1 % (1015 of 1024)	97.7 % (337 of 345)	99.8 % (510 of 511)	100.0 % (168 of 168)
Sidechain	94.2 % (1096 of 1164)	94.2 % (671 of 712)	94.1 % (415 of 441)	90.9 % (10 of 11)
Aromatic	72.7 % (96 of 132)	75.8 % (50 of 66)	68.8 % (44 of 64)	100.0 % (2 of 2)
Methyl	99.4 % (173 of 174)	98.9 % (86 of 87)	100.0 % (87 of 87)

1. delta

GIKQYSQEEL KEMALVEIAH ELFEEHKKPV PFQELLNEIA SLLGVKKEEL GDRIAQFYTD LNIDGRFLAL SDQTWGLRSW YPYDQLDEET QPTVKAKKKK AKKAVEEDLD LDEFEEIDED DLDLDEVEEE LDLEADDFDE EDLDEDDDDL EIEEDIIDED DEDYDDEEEE IK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301 K, pH 6.6

#	Name	Isotope labeling	Concentration
1	delta	[U-100% 13C; U-100% 15N]	0.7 mM
2	D2O	[U-2H]	10 %
3	sodium chloride	natural abundance	10 mM
4	sodium azide	natural abundance	50 uM
5	phosphate buffer	natural abundance	20 mM
6	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	direct	1.0

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	direct	1.0

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	direct	1.0

Referencing offset: 0.08 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	internal	direct	1.0

Referencing offset: 0.3 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M4K, Strand ID: A Detail

Release date

2010-10-04

Citation

Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments
Motakova, V., Novaek, J., Zawadzka-Kazimierczuk, A., Kazimierczuk, K., Zidek, L., Sanderova, H., Krasny, L., Komiski, W., Sklena, V.
J. Biomol. NMR (2010), 48, 169-177, PubMed 20890634 , DOI 10.1007/s10858-010-9447-3 , Abstract

Related entities 1. delta, : 1 : 8 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC