BMRB: 16920

NMR structure of human Tra2beta1 RRM in complex with AAGAAC RNA

Authors

Clery, A., Benderska, N., Jayne, S., Dominguez, C., Stamm, S., Allain, F.H.T.

Assembly

Tra2beta1 RRM in complex with AAGAAC RNA

Entity

1. RNA (polymer, Thiol state: not present), 6 monomers, 1985.226 Da Detail

AAGAAC

2. Tra2beta1 (polymer, Thiol state: all free), 95 monomers, 10926.11 Da Detail

RHVGNRANPD PNCCLGVFGL SLYTTERDLR EVFSKYGPIA DVSIVYDQQS RRSRGFAFVY FENVDDAKEA KERANGMELD GRRIRVDFSI TKRPH

Total weight

12911.336 Da

Max. entity weight

10926.11 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.0 %, Completeness: 78.5 %, Completeness (bb): 74.0 % Detail

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Polymer type: polyribonucleotide polypeptide(L)

	Total	1H	13C	15N
All	78.5 % (941 of 1199)	88.7 % (560 of 631)	61.8 % (290 of 469)	91.9 % (91 of 99)
Backbone	74.0 % (465 of 628)	89.5 % (205 of 229)	56.5 % (174 of 308)	94.5 % (86 of 91)
Sidechain	84.7 % (558 of 659)	88.3 % (355 of 402)	79.5 % (198 of 249)	62.5 % (5 of 8)
Aromatic	67.7 % (84 of 124)	91.9 % (57 of 62)	44.3 % (27 of 61)	0.0 % (0 of 1)
Methyl	95.3 % (82 of 86)	95.3 % (41 of 43)	95.3 % (41 of 43)

1. RNA

AAGAAC

2. Tra2beta1

RHVGNRANPD PNCCLGVFGL SLYTTERDLR EVFSKYGPIA DVSIVYDQQS RRSRGFAFVY FENVDDAKEA KERANGMELD GRRIRVDFSI TKRPH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 5.5, Details Final NMR buffer was: 20mM NaH2PO4, pH 5.5; L-Arg 50mM; L-Glu 50mM

#	Name	Isotope labeling	Type	Concentration
1	Tra2beta1	[U-15N]		0.8 (±0.05) mM
2	Tra2beta1	[U-13C; U-15N]		0.8 (±0.05) mM
3	RNA	natural abundance		0.8 (±0.05) mM
4	NaH2PO4	natural abundance		20 mM
5	L-Arg	natural abundance		50 mM
6	L-Glu	natural abundance		50 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.7 ppm	internal	indirect	0.2514495
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	water	protons	4.7 ppm	internal	indirect	0.101329

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LACS Plot; CB

Referencing offset: -0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.7 ppm	internal	indirect	0.2514495
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	water	protons	4.7 ppm	internal	indirect	0.101329

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.7 ppm	internal	indirect	0.2514495
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	water	protons	4.7 ppm	internal	indirect	0.101329

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Protein Blocks Logo

Calculated from 12 models in PDB: 2KXN, Strand ID: B Detail

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Release date

2011-03-23

Citation

Molecular basis of purine-rich RNA recognition by the human SR-like protein Tra2-β1
Clery, A., Jayne, S., Benderska, N., Dominguez, C., Stamm, S., Allain, F.
Nat. Struct. Mol. Biol. (2011), 18, 443-450, PubMed 21399644 , DOI 10.1038/nsmb.2001 , Abstract

Related entities 2. Tra2beta1, : 1 : 4 : 2 : 154 entities Detail

Interaction partners 2. Tra2beta1, : 1 interactors Detail

Experiments performed 10 experiments Detail