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BMRB: 16934

2KY4

Solution NMR structure of the PBS linker domain of phycobilisome linker polypeptide from Anabaena sp. Northeast Structural Genomics Consortium Target NsR123E

Authors

He, Y., Eletsky, A., Mills, J.L., Lee, D., Ciccosanti, C., Hamilton, K., Acton, T.B., Xiao, R., Everett, J.K., Lee, H., Prestegard, J.H., Montelione, G.T., Szyperski, T.

Assembly

PBS domain

Entity

1. PBS domain (polymer, Thiol state: not present), 149 monomers, 17228.38 Da Detail

MKVFKRVAGI KDKAAIKTLI SAAYRQIFER DIAPYIAQNE FSGWESKLGN GEITVKEFIE GLGYSNLYLK EFYTPYPNTK VIELGTKHFL GRAPIDQAEI RKYNQILATQ GIRAFINALV NSQEYNEVFG EDTVPYRRFP TLEHHHHHH

Formula weight

17228.38 Da

Source organism

Anabaena sp.

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 93.5 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.5 % (1699 of 1818)	93.2 % (883 of 947)	93.3 % (665 of 713)	95.6 % (151 of 158)
Backbone	94.6 % (834 of 882)	94.0 % (284 of 302)	94.7 % (414 of 437)	95.1 % (136 of 143)
Sidechain	92.7 % (997 of 1075)	92.9 % (599 of 645)	92.3 % (383 of 415)	100.0 % (15 of 15)
Aromatic	80.2 % (162 of 202)	83.2 % (84 of 101)	77.0 % (77 of 100)	100.0 % (1 of 1)
Methyl	99.4 % (163 of 164)	98.8 % (81 of 82)	100.0 % (82 of 82)

1. PBS

MKVFKRVAGI KDKAAIKTLI SAAYRQIFER DIAPYIAQNE FSGWESKLGN GEITVKEFIE GLGYSNLYLK EFYTPYPNTK VIELGTKHFL GRAPIDQAEI RKYNQILATQ GIRAFINALV NSQEYNEVFG EDTVPYRRFP TLEHHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	entity	[U-5% 13C; U-100% 15N]	0.975 mM
11	sodium chloride	natural abundance	100 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	10 mM
14	MES	natural abundance	20 mM
15	NaN3	natural abundance	0.02 %
16	DSS	natural abundance	50 uM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Sample #3

Solvent system 82% H2O/18% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
19	entity	[U-5% 13C; U-100% 15N]	0.664 mM
20	sodium chloride	natural abundance	100 mM
21	calcium chloride	natural abundance	5 mM
22	DTT	natural abundance	10 mM
23	MES	natural abundance	20 mM
24	NaN3	natural abundance	0.02 %
25	DSS	natural abundance	50 uM
26	poly ethylene glycol	natural abundance	4 %
27	H2O	natural abundance	82 %
28	D2O	natural abundance	18 %

Sample #4

Solvent system 83% H2O/17% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
29	entity	[U-5% 13C; U-100% 15N]	0.851 mM
30	sodium chloride	natural abundance	100 mM
31	calcium chloride	natural abundance	5 mM
32	DTT	natural abundance	10 mM
33	MES	natural abundance	20 mM
34	NaN3	natural abundance	0.02 %
35	DSS	natural abundance	50 uM
36	poly acrylamide gel	natural abundance	7 %
37	H2O	natural abundance	83 %
38	D2O	natural abundance	17 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 14 models in PDB: 2KY4, Strand ID: A Detail

Release date

2010-07-07

Citation

Solution NMR structure of the PBS linker domain of phycobilisome linker polypeptide from Anabaena sp. Northeast Structural Genomics Consortium Target NsR123E
He, Y., Eletsky, A., Mills, J.L., Lee, D., Ciccosanti, C., Hamilton, K., Acton, T.B., Xiao, R., Everett, J.K., Lee, H., Montelione, G.T., Szyperski, T.
Not known

Related entities 1. PBS domain, : 1 : 1 : 81 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

2 2D 1H-13C CT-HSQC ali

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

3 2D 1H-13C CT-HSQC aro

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

4 2D 1H-13C CT-HSQC (methyl)

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	entity	[U-5% 13C; U-100% 15N]	0.975 mM
11	sodium chloride	natural abundance	100 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	10 mM
14	MES	natural abundance	20 mM
15	NaN3	natural abundance	0.02 %
16	DSS	natural abundance	50 uM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

5 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

6 3D HN(CA)CO

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

7 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

8 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

10 3D HCCH-COSY ali

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

11 3D HCCH-COSY aro

Instrument

Varian INOVA - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

12 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

13 13C/15N-NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	entity	[U-100% 13C; U-100% 15N]	0.95 mM
2	sodium chloride	natural abundance	100 mM
3	calcium chloride	natural abundance	5 mM
4	DTT	natural abundance	10 mM
5	MES	natural abundance	20 mM
6	NaN3	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

14 2D J-modulation 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 82% H2O/18% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
19	entity	[U-5% 13C; U-100% 15N]	0.664 mM
20	sodium chloride	natural abundance	100 mM
21	calcium chloride	natural abundance	5 mM
22	DTT	natural abundance	10 mM
23	MES	natural abundance	20 mM
24	NaN3	natural abundance	0.02 %
25	DSS	natural abundance	50 uM
26	poly ethylene glycol	natural abundance	4 %
27	H2O	natural abundance	82 %
28	D2O	natural abundance	18 %

15 2D J-modulation 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 83% H2O/17% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
29	entity	[U-5% 13C; U-100% 15N]	0.851 mM
30	sodium chloride	natural abundance	100 mM
31	calcium chloride	natural abundance	5 mM
32	DTT	natural abundance	10 mM
33	MES	natural abundance	20 mM
34	NaN3	natural abundance	0.02 %
35	DSS	natural abundance	50 uM
36	poly acrylamide gel	natural abundance	7 %
37	H2O	natural abundance	83 %
38	D2O	natural abundance	17 %

16 2D J-modulation 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	entity	[U-5% 13C; U-100% 15N]	0.975 mM
11	sodium chloride	natural abundance	100 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	10 mM
14	MES	natural abundance	20 mM
15	NaN3	natural abundance	0.02 %
16	DSS	natural abundance	50 uM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

NMR combined restraints 7 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_16934_2ky4.nef
Input source #2: Coordindates	2ky4.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MKVFKRVAGIKDKAAIKTLISAAYRQIFERDIAPYIAQNEFSGWESKLGNGEITVKEFIEGLGYSNLYLKEFYTPYPNTKVIELGTKHFLGRAPIDQAEI
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MKVFKRVAGIKDKAAIKTLISAAYRQIFERDIAPYIAQNEFSGWESKLGNGEITVKEFIEGLGYSNLYLKEFYTPYPNTKVIELGTKHFLGRAPIDQAEI

-------110-------120-------130-------140---------
RKYNQILATQGIRAFINALVNSQEYNEVFGEDTVPYRRFPTLEHHHHHH
|||||||||||||||||||||||||||||||||||||||||||||||||
RKYNQILATQGIRAFINALVNSQEYNEVFGEDTVPYRRFPTLEHHHHHH

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	149	0	0	100.0

Content subtype: combined_16934_2ky4.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1739		96.6 (chain: A, length: 149)
1	Distance restraints	CNS/XPLOR_distance_constraints_6	OK	4417 (1)	noe	96.0 (chain: A, length: 149)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_2	OK	158 (158)	.	64.4 (chain: A, length: 149)
2	Dihedral angle restraints	CNS/XPLOR_dihedral_5	OK	158 (158)	.	64.4 (chain: A, length: 149)
1	RDC restraints	DYANA/DIANA_dipolar_coupling_3	OK	131 (131)	.	51.7 (chain: A, length: 149)
2	RDC restraints	CNS/XPLOR_dipolar_coupling_4	OK	131 (131)	.	51.7 (chain: A, length: 149)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 149, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 912
  - ¹³C chemical shifts: 671
  - ¹⁵N chemical shifts: 156
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
54	THR	HG1	4.745
65	SER	HG	6.608
74	THR	HG1	3.527
86	THR	HG1	4.788
109	THR	HG1	5.174
122	SER	HG	5.433
125	TYR	HH	9.057
138	ARG	HH11	6.695
138	ARG	HH12	6.695
138	ARG	HH21	6.695
138	ARG	HH22	6.695

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	947	901	95.1
¹³C chemical shifts	713	671	94.1
¹⁵N chemical shifts	166	156	94.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	302	289	95.7
¹³C chemical shifts	298	286	96.0
¹⁵N chemical shifts	143	135	94.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	645	612	94.9
¹³C chemical shifts	415	385	92.8
¹⁵N chemical shifts	23	21	91.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	83	83	100.0
¹³C chemical shifts	83	83	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	101	84	83.2
¹³C chemical shifts	100	77	77.0
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_6
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 149, Sequence coverage: 96.0%
- Total number of restraints: 4417
- Weight of restraints: 1.0 (4417)
- Potential type of restraints: square-well-parabolic (4417)
- Range of distance target values: 1.66, 3.42 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 149, Sequence coverage: 64.4%
- Total number of restraints: 158
- Total number of restraints per polymer type: 158
- Weight of restraints: 1.0 (158)
- Potential type of restraints: square-well-parabolic (158)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
- Saveframe: CNS/XPLOR_dihedral_5
  - Status: OK
  - Experiment type: .
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 149, Sequence coverage: 64.4%
  - Total number of restraints: 158
  - Total number of restraints per polymer type: 158
  - Weight of restraints: 1.0 (158)
  - Potential type of restraints: square-well-parabolic (158)
  - Plot of Phi-Psi angle restraints
  - Dihedral angle restraints per residue
    - Chain_ID: A

RDC restraints

Saveframe: DYANA/DIANA_dipolar_coupling_3

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 149, Sequence coverage: 51.7%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MKVFKRVAGIKDKAAIKTLISAAYRQIFERDIAPYIAQNEFSGWESKLGNGEITVKEFIEGLGYSNLYLKEFYTPYPNTKVIELGTKHFLGRAPIDQAEI
  |  |||| |||||| ||  || |   | |||    |  | |||   ||| || ||| || | |  | ||| |   |||| |||||||   | ||||| 
..V..RVAG.KDKAAI.TL..AA.R...E.DIA....Q..F.GWE...GNG.IT.KEF.EG.G.S..Y.KEF.T...NTKV.ELGTKHF...A.IDQAE.
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-------110-------120-------130-------140---------
RKYNQILATQGIRAFINALVNSQEYNEVFGEDTVPYRRFPTLEHHHHHH
|||| || || | |||| |   |   ||     |               
RKYN.IL.TQ.I.AFIN.L...Q...EV.....V               
-------110-------120-------130----

Total number of restraints: 131
Potential type of restraints: undefined (131)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Saveframe: CNS/XPLOR_dipolar_coupling_4

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 149, Sequence coverage: 51.7%

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MKVFKRVAGIKDKAAIKTLISAAYRQIFERDIAPYIAQNEFSGWESKLGNGEITVKEFIEGLGYSNLYLKEFYTPYPNTKVIELGTKHFLGRAPIDQAEI
  |  |||| |||||| ||  || |   | |||    |  | |||   ||| || ||| || | |  | ||| |   |||| |||||||   | ||||| 
..V..RVAG.KDKAAI.TL..AA.R...E.DIA....Q..F.GWE...GNG.IT.KEF.EG.G.S..Y.KEF.T...NTKV.ELGTKHF...A.IDQAE.
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

-------110-------120-------130-------140---------
RKYNQILATQGIRAFINALVNSQEYNEVFGEDTVPYRRFPTLEHHHHHH
|||| || || | |||| |   |   ||     |               
RKYN.IL.TQ.I.AFIN.L...Q...EV.....V               
-------110-------120-------130----

Total number of restraints: 131
Potential type of restraints: undefined (131)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords NESG, PSI, protein structure, solution NMR

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Found 1 Document (0.578 seconds)

BMRB: 16934

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. PBS domain, : 1 : 1 : 81 entities Detail

Experiments performed 16 experiments Detail

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NMR combined restraints 7 contents Detail