BMRB: 16960

Solution structure of GxTX-1E

Authors

Lee, S., Jung, H., Lee, J., Lee, C., Kim, J.

Assembly

Solution structure of GxTX-1E

Entity

1. Solution structure of GxTX-1E (polymer, Thiol state: all disulfide bound), 36 monomers, 3954.640 Da Detail

EGECGGFWWK CGSGKPACCP KYVCSPKWGL CNFPMP

Formula weight

3954.64 Da

Source organism

Chilobrachys guangxiensis

Exptl. method

solution NMR

Refine. method

CYANA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 83.7 %, Completeness (bb): 94.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	83.7 % (185 of 221)	83.7 % (185 of 221)
Backbone	94.5 % (69 of 73)	94.5 % (69 of 73)
Sidechain	78.4 % (116 of 148)	78.4 % (116 of 148)
Aromatic	62.5 % (20 of 32)	62.5 % (20 of 32)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. GxTX-1E

EGECGGFWWK CGSGKPACCP KYVCSPKWGL CNFPMP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 310.000 K, pH 3.500, Details GxTX-1E 10 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	GxTX-1E	natural abundance		10 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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Calculated from 20 models in PDB: 2WH9, Strand ID: A Detail

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Release date

2010-07-25

Citation

Solution structure of GxTX-1E, a high-affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels
Lee, S., Milescu, M., Jung, H., Lee, J., Bae, C., Lee, C., Kim, H., Swartz, K.J., Kim, J.
Biochemistry (2010), 49, 5134-5142, PubMed 20509680 , DOI 10.1021/bi100246u , Abstract

Related entities 1. Solution structure of GxTX-1E, : 1 : 1 : 3 : 74 entities Detail

Experiments performed 3 experiments Detail