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BMRB: 16967

2KYL

Solution structure of MAST2-PDZ complexed with the C-terminus of PTEN

Authors

TERRIEN, E., WOLFF, N., CORDIER, F., SIMENEL, C., LAFON, M., DELEPIERRE, M.

Assembly

MAST2-PDZ/PTEN-C Terminus

Entity

1. MAST2-PDZ (polymer, Thiol state: not present), 96 monomers, 10435.80 Da Detail

GGSMRPPIII HRAGKKYGFT LRAIRVYMGD SDVYTVHHMV WHVEDGGPAS EAGLRQGDLI THVNGEPVHG LVHTEVVELI LKSGNKVAIS TTPLEN

2. PTEN-C Terminus (polymer, Thiol state: not present), 13 monomers, 1557.657 Da Detail

PFDEDQHTQI TKV

Total weight

11993.457 Da

Max. entity weight

10435.8 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.1 %, Completeness: 84.3 %, Completeness (bb): 86.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.3 % (1039 of 1232)	84.1 % (533 of 634)	84.4 % (412 of 488)	85.5 % (94 of 110)
Backbone	86.3 % (554 of 642)	87.1 % (195 of 224)	85.4 % (269 of 315)	87.4 % (90 of 103)
Sidechain	83.0 % (570 of 687)	82.4 % (338 of 410)	84.4 % (228 of 270)	57.1 % (4 of 7)
Aromatic	70.5 % (62 of 88)	70.5 % (31 of 44)	69.8 % (30 of 43)	100.0 % (1 of 1)
Methyl	91.0 % (122 of 134)	91.0 % (61 of 67)	91.0 % (61 of 67)

1. MAST2-PDZ

GGSMRPPIII HRAGKKYGFT LRAIRVYMGD SDVYTVHHMV WHVEDGGPAS EAGLRQGDLI THVNGEPVHG LVHTEVVELI LKSGNKVAIS TTPLEN

2. PTEN-C Terminus

PFDEDQHTQI TKV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.6 mM
2	entity_2	natural abundance	1.2 mM
3	Tris-HCL	natural abundance	50 mM
4	NaCl	natural abundance	150 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2KYL, Strand ID: A, B Detail

Release date

2012-09-19

Citation

Interference with the PTEN-MAST2 interaction by a viral protein leads to cellular relocalization of PTEN
Terrien, E., Chaffotte, A., Lafage, M., Khan, Z., Prehaud, C., Cordier, F., Simenel, C., Delepierre, M., Buc, H., Lafon, M., Wolff, N.
Sci. Signal. (2012), 5, ra58-ra58, PubMed 22894835 , DOI 10.1126/scisignal.2002941 , Abstract

Related entities 1. MAST2-PDZ, : 2 : 174 entities Detail

Related entities 2. PTEN-C Terminus, : 1 : 3 : 3 : 1 : 2 entities Detail

Interaction partners 1. MAST2-PDZ, : 19 interactors Detail

More details for 19 interactors identified by 10 citations

Interaction partners 2. PTEN-C Terminus, : 10 : 1 interactors Detail

More details for 11 interactors identified by 9 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC