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BMRB: 16977

2L8K

Resonance assignment of nsp7a from arterivirus

Authors

Conte, M.R., Gaudin, C., Manolaridis, I., Tucker, P.A.

Assembly

nsp7a

Entity

1. nsp7a (polymer, Thiol state: all free), 123 monomers, 13473.29 Da Detail

GLTATLAALT DDDFQFLSDV LDCRAVRSAM NLRAALTSFQ VAQYRNILNA SLQVDRDAAR SRRLMAKLAD FAVEQEVTAG DRVVVIDGLD RMAHFKDDLV LVPLTTKVVG GSRCTICDVV KEE

Formula weight

13473.29 Da

Source organism

Equine arteritis virus

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.9 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.9 % (1254 of 1364)	89.8 % (625 of 696)	93.5 % (503 of 538)	96.9 % (126 of 130)
Backbone	96.6 % (711 of 736)	96.4 % (241 of 250)	96.7 % (352 of 364)	96.7 % (118 of 122)
Sidechain	88.3 % (659 of 746)	86.1 % (384 of 446)	91.4 % (267 of 292)	100.0 % (8 of 8)
Aromatic	56.5 % (35 of 62)	61.3 % (19 of 31)	51.6 % (16 of 31)
Methyl	96.7 % (174 of 180)	95.6 % (86 of 90)	97.8 % (88 of 90)

1. nsp7a

GLTATLAALT DDDFQFLSDV LDCRAVRSAM NLRAALTSFQ VAQYRNILNA SLQVDRDAAR SRRLMAKLAD FAVEQEVTAG DRVVVIDGLD RMAHFKDDLV LVPLTTKVVG GSRCTICDVV KEE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

LACS Plot; CA

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.24 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.26 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L8K, Strand ID: A Detail

Release date

2010-08-18

Citation

Resonance assignment of nsp7α from arterivirus
Gaudin, C., Manolaridis, I., Tucker, P.A., Conte, M.R.
Biomol. NMR Assign. (2011), 5, 23-25, PubMed 20703834 , DOI 10.1007/s12104-010-9258-1 , Abstract

Related entities 1. nsp7a, : 1 : 1 : 8 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

4 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

5 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

6 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

7 3D HN(CO)CA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	nsp7a	[U-100% 13C; U-100% 15N]		0.4 ~ 0.6 mM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %
4	potassium phosphate	natural abundance		20 mM
5	NaCl	natural abundance		50 nM

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