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Found 1 Document (1.177 seconds)

BMRB: 16996

2KZ3

Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Rad51D from 1 to 83

Authors

Choi, B., Kim, Y.

Assembly

human Rad51D N

Entity

1. human Rad51D N (polymer, Thiol state: all free), 83 monomers, 9060.583 Da Detail

MGVLRVGLCP GLTEEMIQLL RSHRIKTVVD LVSADLEEVA QKCGLSYKAL VALRRVLLAQ FSAFPVNGAD LYEELKTSTA ILS

Formula weight

9060.583 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 91.1 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.1 % (868 of 953)	92.0 % (450 of 489)	88.9 % (337 of 379)	95.3 % (81 of 85)
Backbone	97.0 % (479 of 494)	96.4 % (163 of 169)	96.7 % (236 of 244)	98.8 % (80 of 81)
Sidechain	86.8 % (466 of 537)	89.7 % (287 of 320)	83.6 % (178 of 213)	25.0 % (1 of 4)
Aromatic	60.0 % (24 of 40)	80.0 % (16 of 20)	40.0 % (8 of 20)
Methyl	87.9 % (116 of 132)	93.9 % (62 of 66)	81.8 % (54 of 66)

1. human Rad51D N

MGVLRVGLCP GLTEEMIQLL RSHRIKTVVD LVSADLEEVA QKCGLSYKAL VALRRVLLAQ FSAFPVNGAD LYEELKTSTA ILS

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details The N-terminal domain of human Rad51D in 25mM Tris-HCl (pH7.5), NaCl 100mM, 1mM DTT and 0.02% Sodium azide

#	Name	Isotope labeling	Concentration
1	human Rad51D_N	[U-100% 15N]	1 mM
2	DTT	natural abundance	1 mM
3	sodium azide	natural abundance	0.02 %
4	Tris-HCl	natural abundance	25 mM
5	NaCl	natural abundance	100 mM
6	D2O	natural abundance	100 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details [1mM]human Rad51D_N in 25mM Tris-HCl (pH7.5) 100mM NaCl,1mM DTT and 0.02% Sodium azide

#	Name	Isotope labeling	Concentration
7	human Rad51D_N	[U-99% 13C; U-99% 15N]	1 mM
8	TRIS	natural abundance	25 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.02 %
12	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details 15N sample

#	Name	Isotope labeling	Concentration
13	human Rad51D_N	[U-99% 15N]	1 mM
14	TRIS	natural abundance	25 mM
15	sodium chloride	natural abundance	100 mM
16	DTT	natural abundance	1 mM
17	sodium azide	natural abundance	0.02 %
18	D2O	natural abundance	90 %
19	H2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KZ3, Strand ID: A Detail

Release date

2011-05-31

Citation

Structural and functional characterization of the N-terminal domain of human Rad51D
Kim, Y., Choi, B.
Int. J. Biochem. Cell Biol. (2011), 43, 416-422, PubMed 21111057 , DOI 10.1016/j.biocel.2010.11.014 , Abstract

Related entities 1. human Rad51D N, : 1 : 2 : 22 entities Detail

Interaction partners 1. human Rad51D N, : 27 interactors Detail

More details for 27 interactors identified by 7 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details 15N sample

#	Name	Isotope labeling	Concentration
13	human Rad51D_N	[U-99% 15N]	1 mM
14	TRIS	natural abundance	25 mM
15	sodium chloride	natural abundance	100 mM
16	DTT	natural abundance	1 mM
17	sodium azide	natural abundance	0.02 %
18	D2O	natural abundance	90 %
19	H2O	natural abundance	10 %

2 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details 15N sample

#	Name	Isotope labeling	Concentration
13	human Rad51D_N	[U-99% 15N]	1 mM
14	TRIS	natural abundance	25 mM
15	sodium chloride	natural abundance	100 mM
16	DTT	natural abundance	1 mM
17	sodium azide	natural abundance	0.02 %
18	D2O	natural abundance	90 %
19	H2O	natural abundance	10 %

3 3D 1H-15N TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details 15N sample

#	Name	Isotope labeling	Concentration
13	human Rad51D_N	[U-99% 15N]	1 mM
14	TRIS	natural abundance	25 mM
15	sodium chloride	natural abundance	100 mM
16	DTT	natural abundance	1 mM
17	sodium azide	natural abundance	0.02 %
18	D2O	natural abundance	90 %
19	H2O	natural abundance	10 %

4 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details [1mM]human Rad51D_N in 25mM Tris-HCl (pH7.5) 100mM NaCl,1mM DTT and 0.02% Sodium azide

#	Name	Isotope labeling	Concentration
7	human Rad51D_N	[U-99% 13C; U-99% 15N]	1 mM
8	TRIS	natural abundance	25 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.02 %
12	D2O	natural abundance	100 %

5 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details [1mM]human Rad51D_N in 25mM Tris-HCl (pH7.5) 100mM NaCl,1mM DTT and 0.02% Sodium azide

#	Name	Isotope labeling	Concentration
7	human Rad51D_N	[U-99% 13C; U-99% 15N]	1 mM
8	TRIS	natural abundance	25 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	1 mM
11	sodium azide	natural abundance	0.02 %
12	D2O	natural abundance	100 %

6 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 287 K, pH 7.5, Details The N-terminal domain of human Rad51D in 25mM Tris-HCl (pH7.5), NaCl 100mM, 1mM DTT and 0.02% Sodium azide

#	Name	Isotope labeling	Concentration
1	human Rad51D_N	[U-100% 15N]	1 mM
2	DTT	natural abundance	1 mM
3	sodium azide	natural abundance	0.02 %
4	Tris-HCl	natural abundance	25 mM
5	NaCl	natural abundance	100 mM
6	D2O	natural abundance	100 %

7 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	human Rad51D_N	[U-100% 15N]	1 mM
2	DTT	natural abundance	1 mM
3	sodium azide	natural abundance	0.02 %
4	Tris-HCl	natural abundance	25 mM
5	NaCl	natural abundance	100 mM
6	D2O	natural abundance	100 %

8 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	human Rad51D_N	[U-100% 15N]	1 mM
2	DTT	natural abundance	1 mM
3	sodium azide	natural abundance	0.02 %
4	Tris-HCl	natural abundance	25 mM
5	NaCl	natural abundance	100 mM
6	D2O	natural abundance	100 %

9 3D H(CCO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	human Rad51D_N	[U-100% 15N]	1 mM
2	DTT	natural abundance	1 mM
3	sodium azide	natural abundance	0.02 %
4	Tris-HCl	natural abundance	25 mM
5	NaCl	natural abundance	100 mM
6	D2O	natural abundance	100 %

10 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	human Rad51D_N	[U-100% 15N]	1 mM
2	DTT	natural abundance	1 mM
3	sodium azide	natural abundance	0.02 %
4	Tris-HCl	natural abundance	25 mM
5	NaCl	natural abundance	100 mM
6	D2O	natural abundance	100 %

nullKeywords Protein, Rad51D

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Found 1 Document (1.177 seconds)

BMRB: 16996

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. human Rad51D N, : 1 : 2 : 22 entities Detail

Interaction partners 1. human Rad51D N, : 27 interactors Detail

Experiments performed 10 experiments Detail

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