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BMRB: 16999

2KZ6

Solution structure of protein CV0426 from Chromobacterium violaceum. NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET CVT2.

Authors

Lemak, A., Yee, A., Lee, H., Semesi, A., Prestegard, J., Montelione, G., Arrowsmith, C.

Assembly

CV0426

Entity

1. CV0426 (polymer, Thiol state: all free), 102 monomers, 11598.97 Da Detail

QGMLLHSVET PRGEILNVSE QEARDVFGAS EQAIADARKA TILQTLRIER DERLRACDWT QVQDVVLTAD QKATWAKYRQ ALRDLPETVT DLSQIVWPQL PV

Formula weight

11598.97 Da

Source organism

Chromobacterium violaceum

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 84.8 %, Completeness (bb): 86.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.8 % (1006 of 1186)	85.4 % (526 of 616)	83.8 % (384 of 458)	85.7 % (96 of 112)
Backbone	86.6 % (523 of 604)	88.2 % (179 of 203)	85.8 % (260 of 303)	85.7 % (84 of 98)
Sidechain	84.0 % (572 of 681)	84.0 % (347 of 413)	83.9 % (213 of 254)	85.7 % (12 of 14)
Aromatic	75.9 % (44 of 58)	82.8 % (24 of 29)	65.4 % (17 of 26)	100.0 % (3 of 3)
Methyl	85.5 % (118 of 138)	85.5 % (59 of 69)	85.5 % (59 of 69)

1. cv0426

QGMLLHSVET PRGEILNVSE QEARDVFGAS EQAIADARKA TILQTLRIER DERLRACDWT QVQDVVLTAD QKATWAKYRQ ALRDLPETVT DLSQIVWPQL PV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	cv0426	[U-13C; U-15N]	0.5 mM
2	MOPS	natural abundance	10 mM
3	sodium chloride	natural abundance	450 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	10 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	10 mM
8	D2O	natural abundance	10 %
9	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KZ6, Strand ID: A Detail

Release date

2010-07-06

Citation

Solution structure of protein CV0426 from Chromobacterium violaceum
Lemak, A., Yee, A., Lee, H., Semesi, A., Prestegard, J., Montelione, G., Arrowsmith, C.
Not known

Related entities 1. CV0426, : 1 entities Detail

Experiments performed 10 experiments Detail

1 3D HNCO