BMRB: 17000

Structure of E1-69 of Yeast V-ATPase

Authors

Sankaranarayanan, R., Gruber, G.

Assembly

E1-69 of Yeast V-ATPase

Entity

1. E1-69 of Yeast V-ATPase (polymer, Thiol state: not present), 69 monomers, 7895.838 Da Detail

MASAITALTP NQVNDELNKM QAFIRKEAEE KAKEIQLKAD QEYEIEKTNI VRNETNNIDG NFKSKLKKA

Formula weight

7895.838 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 77.8 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.8 % (648 of 833)	82.8 % (366 of 442)	68.5 % (213 of 311)	86.3 % (69 of 80)
Backbone	98.5 % (406 of 412)	97.8 % (135 of 138)	98.5 % (203 of 206)	100.0 % (68 of 68)
Sidechain	63.2 % (309 of 489)	76.0 % (231 of 304)	44.5 % (77 of 173)	8.3 % (1 of 12)
Aromatic	28.6 % (8 of 28)	57.1 % (8 of 14)	0.0 % (0 of 14)
Methyl	62.5 % (45 of 72)	97.2 % (35 of 36)	27.8 % (10 of 36)

1. entity

MASAITALTP NQVNDELNKM QAFIRKEAEE KAKEIQLKAD QEYEIEKTNI VRNETNNIDG NFKSKLKKA

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-100% 13C; U-100% 15N]		2 mM
2	Na2PO4	natural abundance		25 mM
3	NaCl	natural abundance		200 mM
4	EDTA	natural abundance		5 mM
5	H2O	natural abundance		93 %
6	D2O	natural abundance		7 %

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.94 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2KZ9, Strand ID: A Detail

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Release date

2011-03-23

Citation

NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase
Rishikesan, S., Thaker, Y.R., Gruber, G.
J. Bioenerg. Biomembr. (2011), 43, 187-193, PubMed 21399923 , DOI 10.1007/s10863-011-9342-y , Abstract

Related entities 1. E1-69 of Yeast V-ATPase, : 1 : 12 : 32 entities Detail

Interaction partners 1. E1-69 of Yeast V-ATPase, : 33 interactors Detail

Experiments performed 6 experiments Detail