BMRB: 17001

ASIP(80-132, P103A, P105A, Q115Y, S124Y)

Authors

Patel, M.P., Cribb Fabersunne, C.S., Yang, Y., Kaelin, C.B., Barsh, G.S., Millhauser, G.L.

Assembly

ASIP(80-132, P103A, P105A, Q115Y, S124Y)

Entity

1. ASIP(80-132, P103A, P105A, Q115Y, S124Y) (polymer, Thiol state: all disulfide bound), 53 monomers, 5762.848 Da Detail

KKVVRPRTPL SAPCVATRNS CKPAAAACCD PCASCYCRFF RSACYCRVLS LNC

Formula weight

5762.848 Da

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 85.1 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.1 % (257 of 302)	85.1 % (257 of 302)
Backbone	97.0 % (98 of 101)	97.0 % (98 of 101)
Sidechain	79.1 % (159 of 201)	79.1 % (159 of 201)
Aromatic	88.9 % (16 of 18)	88.9 % (16 of 18)
Methyl	100.0 % (24 of 24)	100.0 % (24 of 24)

1. ASIP(80-132, P103A, P105A, Q115Y, S124Y)

KKVVRPRTPL SAPCVATRNS CKPAAAACCD PCASCYCRFF RSACYCRVLS LNC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details samples was brought to pH 5 using NaOD

#	Name	Isotope labeling	Type	Concentration
1	ASIP(80-132, P103A, P105A, Q115Y, S124Y)	natural abundance		1-2 mM
2	D2O	natural abundance		10 %
3	H2O	natural abundance		90 %
4	acetic acid	[U-100% 2H]		50 mM
5	sodium azide	natural abundance		.1 % w/v
6	TSP	natural abundance		200 uM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details Deuterium Exchange Experiments, samples was brought to pH 4 using NaOD

#	Name	Isotope labeling	Type	Concentration
7	ASIP(80-132, P103A, P105A, Q115Y, S124Y)	natural abundance		1-2 mM
8	D2O	[U-100% 2H]		100 %
9	acetic acid	[U-100% 2H]		50 mM
10	sodium azide	natural abundance		.1 % w/v
11	TSP	natural abundance		200 uM

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Protein Blocks Logo

Calculated from 20 models in PDB: 2KZA, Strand ID: A Detail

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Release date

2010-09-23

Citation

Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism
Patel, M.P., Cribb Fabersunne, C.S., Yang, Y., Kaelin, C.B., Barsh, G.S., Millhauser, G.L.
J. Mol. Biol. (2010), 404, 45-55, PubMed 20831872 , DOI 10.1016/j.jmb.2010.08.054 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. ASIP(80-132, P103A, P105A, Q115Y, S124Y), : 1 : 6 : 25 entities Detail

Experiments performed 4 experiments Detail