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BMRB: 17006

2KZB

Solution structure of alpha-mannosidase binding domain of Atg19

Authors

Watanabe, Y., Noda, N., Kumeta, H., Suzuki, K., Ohsumi, Y., Inagaki, F.

Assembly

Atg19

Entity

1. Atg19 (polymer, Thiol state: all free), 118 monomers, 13375.93 Da Detail

GPHMVEPPNE RSLQITMNQR DNSLYFQLFN NTNSVLAGNC KLKFTDAGDK PTTQIIDMGP HEIGIKEYKE YRYFPYALDL EAGSTIEIEN QYGEVIFLGK YGSSPMINLR PPSRLSAE

Formula weight

13375.93 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 92.8 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.8 % (1295 of 1396)	95.1 % (697 of 733)	88.9 % (480 of 540)	95.9 % (118 of 123)
Backbone	94.1 % (649 of 690)	95.3 % (225 of 236)	92.8 % (320 of 345)	95.4 % (104 of 109)
Sidechain	92.3 % (752 of 815)	95.0 % (472 of 497)	87.5 % (266 of 304)	100.0 % (14 of 14)
Aromatic	71.9 % (82 of 114)	93.0 % (53 of 57)	50.9 % (29 of 57)
Methyl	96.4 % (106 of 110)	96.4 % (53 of 55)	96.4 % (53 of 55)

1. Atg19

GPHMVEPPNE RSLQITMNQR DNSLYFQLFN NTNSVLAGNC KLKFTDAGDK PTTQIIDMGP HEIGIKEYKE YRYFPYALDL EAGSTIEIEN QYGEVIFLGK YGSSPMINLR PPSRLSAE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	100 mM
3	DTT	natural abundance	2 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.77 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KZB, Strand ID: A Detail

Release date

2010-08-11

Citation

Selective transport of alpha-mannosidase by autophagic pathways: structural basis for cargo recognition by Atg19 and Atg34
Watanabe, Y., Noda, N.N., Kumeta, H., Suzuki, K., Ohsumi, Y., Inagaki, F.
J. Biol. Chem. (2010), 285, 30026-30033, PubMed 20659891 , DOI 10.1074/jbc.M110.143545 , Abstract

Related entities 1. Atg19, : 1 : 2 : 4 entities Detail

Interaction partners 1. Atg19, : 29 interactors Detail

More details for 29 interactors identified by 9 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC