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BMRB: 17017

2KZR

Solution NMR Structure of (Ubiquitin thioesterase OTU1 EC3.1.2.-) from Mus musculus, Northeast Structural Genomics Consortium Target MmT2A

Authors

Chitayat, S., Gutmanas, A., Lemak, A., Yee, A., Bezsonova, I., Wu, B., Doherty, R.S., Semesi, A., Montelione, G.T., Arrowsmith, C.H., Dhe-Paganon, S.

Assembly

MmT2A

Entity

1. MmT2A (polymer, Thiol state: all free), 86 monomers, 9352.610 Da Detail

WRVRCKAKGG THLLQGLSSR TRLRELQGQI AAITGIAPGS QRILVGYPPE CLDLSDRDIT LGDLPIQSGD MLIVEEDQTR PKASPS

Formula weight

9352.61 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 94.5 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (918 of 971)	94.3 % (481 of 510)	93.6 % (350 of 374)	100.0 % (87 of 87)
Backbone	96.2 % (485 of 504)	97.7 % (171 of 175)	94.0 % (234 of 249)	100.0 % (80 of 80)
Sidechain	93.2 % (507 of 544)	92.5 % (310 of 335)	94.1 % (190 of 202)	100.0 % (7 of 7)
Aromatic	75.0 % (18 of 24)	83.3 % (10 of 12)	63.6 % (7 of 11)	100.0 % (1 of 1)
Methyl	98.1 % (102 of 104)	98.1 % (51 of 52)	98.1 % (51 of 52)

1. MmT2A

WRVRCKAKGG THLLQGLSSR TRLRELQGQI AAITGIAPGS QRILVGYPPE CLDLSDRDIT LGDLPIQSGD MLIVEEDQTR PKASPS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.1 mM [U-100% 13C; U-100% 15N] SR232, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	MmT2A	[U-100% 13C; U-100% 15N]	1.1 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.99 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KZR, Strand ID: A Detail

Release date

2010-07-25

Related entities 1. MmT2A, : 1 : 4 : 21 entities Detail

Interaction partners 1. MmT2A, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC