BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.5 % (1104 of 1156)	96.5 % (587 of 608)	93.8 % (421 of 449)	97.0 % (96 of 99)
Backbone	96.2 % (537 of 558)	95.7 % (179 of 187)	96.4 % (270 of 280)	96.7 % (88 of 91)
Sidechain	95.4 % (658 of 690)	96.9 % (408 of 421)	92.7 % (242 of 261)	100.0 % (8 of 8)
Aromatic	81.6 % (80 of 98)	100.0 % (49 of 49)	63.3 % (31 of 49)
Methyl	99.0 % (99 of 100)	98.0 % (49 of 50)	100.0 % (50 of 50)

1. DAXX

GSHMGKKCYK LENEKLFEEF LELCKMQTAD HPEVVPFLYN RQQRAHSLFL ASAEFCNILS RVLSRARSRP AKLYVYINEL CTVLKAHSAK KKLN

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	DAXX	[U-10% 13C]	0.8 mM
8	potassium phosphate	natural abundance	10 mM
9	KCl	natural abundance	100 mM
10	EDTA	natural abundance	0.1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 2KZS, Strand ID: A Detail

Heteronucl. T₁

83 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Heteronucl. T₂

83 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Heteronucl. NOE

83 NOE values in 1 lists
Value type peak height, Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Heteronucl. T₁/T₂

83 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

¹H exchange rate

25 k_ex values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Release date

2011-03-06

Citation

Structural characterization of the DAXX N-terminal helical bundle domain and its complex with Rassf1C
Escobar-Cabrera, E., Lau, D.K.W., Giovinazzi, S.R., Alexander, I.M., McIntosh, L.P.
Structure (2010), 18, 1642-1653, PubMed 21134643 , DOI 10.1016/j.str.2010.09.016 , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 17019 released on 2011-03-06
    Title DAXX helical bundle (DHB) domain / Rassf1C complex
  Swiss-Prot: Q9NS23 released on 2004-07-19
    Title RASF1_HUMAN Entity Ras association domain-containing protein 1
  Swiss-Prot: Q9UER7 released on 2002-11-01
    Title DAXX_HUMAN Entity Death domain-associated protein 6

Related entities 1. DAXX helical bundle, : 1 : 1 : 5 : 8 entities Detail

Interaction partners 1. DAXX helical bundle, : 79 interactors Detail

More details for 79 interactors identified by 46 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

2 3D HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

3 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

5 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

6 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

7 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

8 3D 1H-15N TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

9 3D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

10 3D H(CC)(CO)NH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

11 2D (HB)CB(CGCD)HD

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

12 2D (HB)CBCGCDCE)HE

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

13 2D HMBC

Instrument

Varian Unity - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

14 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	DAXX	[U-10% 13C]	0.8 mM
8	potassium phosphate	natural abundance	10 mM
9	KCl	natural abundance	100 mM
10	EDTA	natural abundance	0.1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

15 CT methyl-methyl-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

16 CT amide-methyl-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

17 2D IPAP-HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	DAXX	[U-10% 13C]	0.8 mM
8	potassium phosphate	natural abundance	10 mM
9	KCl	natural abundance	100 mM
10	EDTA	natural abundance	0.1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

18 2D heteronuclear NOE

Instrument

Varian Unity - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

19 2D T1

Instrument

Varian Unity - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

20 2D T2

Instrument

Varian Unity - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]	1.2 mM
2	potassium phosphate	natural abundance	10 mM
3	KCl	natural abundance	100 mM
4	EDTA	natural abundance	0.1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

nullKeywords DAXX, Helix bundle, DAXX helical bundle (DHB) domain

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Found 1 Document (2.865 seconds)

BMRB: 17018

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

Related entities 1. DAXX helical bundle, : 1 : 1 : 5 : 8 entities Detail

Interaction partners 1. DAXX helical bundle, : 79 interactors Detail

Experiments performed 20 experiments Detail

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