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BMRB: 17019

2KZU

DAXX helical bundle (DHB) domain / Rassf1C complex

Authors

Escobar-Cabrera, E., McIntosh, L.P.

Assembly

DAXX / Rassf1C complex

Entity

1. DAXX (polymer, Thiol state: all free), 94 monomers, 10930.67 Da Detail

GSHMGKKCYK LENEKLFEEF LELCKMQTAD HPEVVPFLYN RQQRAHSLFL ASAEFCNILS RVLSRARSRP AKLYVYINEL CTVLKAHSAK KKLN

2. Rassf1C (polymer, Thiol state: not present), 18 monomers, 2148.156 Da Detail

GSQEDSDSEL EQYFTARW

Total weight

13078.826 Da

Max. entity weight

10930.67 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 88.3 %, Completeness (bb): 83.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.3 % (1202 of 1362)	95.5 % (683 of 715)	76.7 % (404 of 527)	95.8 % (115 of 120)
Backbone	83.3 % (555 of 666)	96.4 % (216 of 224)	70.6 % (235 of 333)	95.4 % (104 of 109)
Sidechain	93.8 % (755 of 805)	95.1 % (467 of 491)	91.4 % (277 of 303)	100.0 % (11 of 11)
Aromatic	82.8 % (106 of 128)	100.0 % (64 of 64)	65.1 % (41 of 63)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. DAXX

GSHMGKKCYK LENEKLFEEF LELCKMQTAD HPEVVPFLYN RQQRAHSLFL ASAEFCNILS RVLSRARSRP AKLYVYINEL CTVLKAHSAK KKLN

2. Rassf1C

GSQEDSDSEL EQYFTARW

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

Protein Blocks Logo

Calculated from 25 models in PDB: 2KZU, Strand ID: A, B Detail

Release date

2011-03-06

Citation

Structural characterization of the DAXX N-terminal helical bundle domain and its complex with Rassf1C
Escobar-Cabrera, E., Lau, D.K.W., Giovinazzi, S.R., Alexander, I.M., McIntosh, L.P.
Structure (2010), 18, 1642-1653, PubMed 21134643 , DOI 10.1016/j.str.2010.09.016 , Abstract

Related entities 1. DAXX, : 2 : 8 entities Detail

Related entities 2. Rassf1C, : 1 entities Detail

Interaction partners 1. DAXX, : 79 interactors Detail

More details for 79 interactors identified by 46 citations

Experiments performed 23 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

2 2D 1H-15N HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

3 3D CBCA(CO)NH

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

4 3D HNCACB

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

5 3D CBCA(CO)NH

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

6 3D HCCH-TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

7 2D 1H-13C HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

8 (H)CC(CO)NH-TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

9 HCC(CO)NH-TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

10 (HB)CB(CGCD)HD

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

11 (HB)CB(CGCDCE)HE

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

12 3D 1H-15N NOESY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

13 3D 1H-13C NOESY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

14 13C/15N isotope-filtered NOESY-HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAXX	[U-100% 13C; U-100% 15N]		1.1 mM
2	Rassf1C	natural abundance		1.65 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

15 13C/15N isotope-filtered NOESY-HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

16 3D 1H-15N NOESY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

17 3D 1H-13C NOESY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

18 2D 1H-13C HSQC

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

19 (H)CC(CO)NH-TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

20 HCC(CO)NH-TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

21 (HB)CB(CGCD)HD

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

22 (HB)CB(CGCD)HD-TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

23 3D 1H-15N TOCSY

Instrument

Varian Unity - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
5	DAXX	natural abundance		1.8 mM
6	Rassf1C	[U-100% 13C; U-100% 15N]		1.2 mM
7	H2O	natural abundance		95 %
8	D2O	natural abundance		5 %

nullKeywords complex, DAXX, Helical bundle, mitosis, Rassf1C, DAXX helical bundle (DHB) domain