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BMRB: 17023

2KZY

Solution NMR structure of the ZNF216 A20 zinc finger

Authors

Garner, T.P., Long, J.E., Searle, M.S., Layfield, R.

Assembly

Znf A20

Entity

1. ZNF216-A20 (polymer, Thiol state: all other bound), 62 monomers, 6533.223 Da Detail

GSMAQETNQT PGPMLCSTGC GFYGNPRTNG MCSVCYKEHL QRQQNSGRMS PMGTASGSNS PT

2. ZN (non-polymer, Thiol state: not present), 65.409 Da

Total weight

6598.6323 Da

Max. entity weight

6533.223 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS16:SG	2:ZN1:ZN
2	na	sing	1:CYS20:SG	2:ZN1:ZN
3	na	sing	1:CYS32:SG	2:ZN1:ZN
4	na	sing	1:CYS35:SG	2:ZN1:ZN

Source organism

Rattus norvegicus

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.2 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.2 % (524 of 653)	73.3 % (252 of 344)	85.1 % (206 of 242)	98.5 % (66 of 67)
Backbone	93.6 % (339 of 362)	85.2 % (109 of 128)	98.3 % (174 of 177)	98.2 % (56 of 57)
Sidechain	69.2 % (238 of 344)	66.2 % (143 of 216)	72.0 % (85 of 118)	100.0 % (10 of 10)
Aromatic	50.0 % (15 of 30)	100.0 % (15 of 15)	0.0 % (0 of 15)
Methyl	92.9 % (26 of 28)	92.9 % (13 of 14)	92.9 % (13 of 14)

1. ZNF216-A20

GSMAQETNQT PGPMLCSTGC GFYGNPRTNG MCSVCYKEHL QRQQNSGRMS PMGTASGSNS PT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details 50uM ZnCl2 used 0.1 mM DSS used for referencing

#	Name	Isotope labeling	Concentration
1	ZNF216-A20	natural abundance	1 (±0.1) mM
2	ZnCl2	natural abundance	50 (±0.2) uM
3	DSS	natural abundance	0.1 (±0.01) mM
4	TRIS	natural abundance	5 (±0.5) mM
5	sodium chloride	natural abundance	50 (±5.0) mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details 50 uM ZnCl2 added 0.1 mM DSS added

#	Name	Isotope labeling	Concentration
8	ZNF216-A20	[U-100% 15N]	1.2 (±0.1) mM
9	ZnCl2	natural abundance	50 (±0.2) uM
10	DSS	natural abundance	0.1 (±0.01) mM
11	TRIS	natural abundance	5 (±0.5) mM
12	sodium chloride	natural abundance	50 (±5.0) mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details 50 uM ZnCl2 added 0.1 mM DSS used

#	Name	Isotope labeling	Concentration
15	ZNF216-A20	[U-100% 13C; U-100% 15N]	0.8 (±0.2) mM
16	ZnCl2	natural abundance	50 (±0.2) uM
17	DSS	natural abundance	0.1 (±0.01) mM
18	TRIS	natural abundance	5 (±0.5) mM
19	sodium chloride	natural abundance	50 (±5.0) mM
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.05) K, pH 7 (±0.1), Details Sample 2 soaked in 7% PAG gel

#	Name	Isotope labeling	Concentration
22	ZNF216-A20	[U-100% 15N]	1.2 (±0.1) mM
23	ZnCl2	natural abundance	50 (±0.2) uM
24	DSS	natural abundance	0.1 (±0.01) mM
25	TRIS	natural abundance	5 (±0.5) mM
26	sodium chloride	natural abundance	50 (±5.0) mM
27	Polyacrylamide gel	natural abundance	7 (±0.7) %
28	H2O	natural abundance	90 %
29	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2KZY, Strand ID: A Detail

Release date

2012-08-01

Citation

Co-localisation of ubiquitin receptors ZNF216 and p62 in a ubiquitin-mediated ternary complex
Garner, T.P., Strachan, J., Long, J.E., Layfield, R., Searle, M.S.
Nat. Struct. Biol.

Related entities 1. ZNF216-A20, : 1 : 1 : 6 : 22 entities Detail

Interaction partners 1. ZNF216-A20, : 8 interactors Detail

More details for 8 interactors identified by 6 citations