BMRBj - BMREntryMaster

Found 1 Document (0.894 seconds)

BMRB: 17028

2L04

The Solution Structure of the C-terminal Ig-like Domain of the Bacteriophage Lambda Tail Tube Protein

Authors

Pell, L.G., Gasmi-Seabrook, G.M.C., Donaldson, L.W., Howell, P., Davidson, A.R., Maxwell, K.L.

Assembly

Bacteriophage Lambda Tail Tube-gpV-C

Entity

1. Bacteriophage Lambda Tail Tube-gpV-C (polymer, Thiol state: not present), 91 monomers, 8875.969 Da Detail

GAMAAATGMT VTPASTSVVK GQSTTLTVAF QPEGVTDKSF RAVSADKTKA TVSVSGMTIT VNGVAAGKVN IPVVSGNGEF AAVAEITVTA S

Formula weight

8875.969 Da

Source organism

Escherichia virus Lambda

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.5 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.5 % (876 of 917)	96.0 % (437 of 455)	94.0 % (347 of 369)	98.9 % (92 of 93)
Backbone	97.4 % (526 of 540)	98.9 % (186 of 188)	95.8 % (253 of 264)	98.9 % (87 of 88)
Sidechain	94.1 % (432 of 459)	94.4 % (252 of 267)	93.6 % (175 of 187)	100.0 % (5 of 5)
Aromatic	60.0 % (18 of 30)	66.7 % (10 of 15)	53.3 % (8 of 15)
Methyl	95.5 % (128 of 134)	95.5 % (64 of 67)	95.5 % (64 of 67)

1. gpV-C

GAMAAATGMT VTPASTSVVK GQSTTLTVAF QPEGVTDKSF RAVSADKTKA TVSVSGMTIT VNGVAAGKVN IPVVSGNGEF AAVAEITVTA S

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	200 mM
8	D20	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.68 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L04, Strand ID: A Detail

Release date

2010-09-23

Citation

The solution structure of the C-terminal Ig-like domain of the bacteriophage λ tail tube protein
Pell, L.G., Gasmi-Seabrook, G.M.C., Morais, M., Neudecker, P., Kanelis, V., Bona, D., Donaldson, L.W., Edwards, A.M., Howell, P., Davidson, A.R., Maxwell, K.L.
J. Mol. Biol. (2010), 403, 468-479, PubMed 20826161 , DOI 10.1016/j.jmb.2010.08.044 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P03733 released on 1986-07-21
Title TUBE_LAMBD Entity Tail tube protein

Related entities 1. Bacteriophage Lambda Tail Tube-gpV-C, : 1 : 1 : 8 entities Detail

Interaction partners 1. Bacteriophage Lambda Tail Tube-gpV-C, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

3 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

4 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	200 mM
8	D20	natural abundance	100 %

5 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

6 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

7 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

8 3D 1H-13C NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
6	sodium phosphate	natural abundance	50 mM
7	sodium chloride	natural abundance	200 mM
8	D20	natural abundance	100 %

9 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	gpV-C	15N, 13C	0.71 ~ 2.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	200 mM
4	H20	natural abundance	90 %
5	D20	natural abundance	10 %

nullKeywords bacteriophage lambda, gpV, phage tail, Ig-like domain

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.894 seconds)

BMRB: 17028

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Bacteriophage Lambda Tail Tube-gpV-C, : 1 : 1 : 8 entities Detail

Interaction partners 1. Bacteriophage Lambda Tail Tube-gpV-C, : 6 interactors Detail

Experiments performed 9 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample