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BMRB: 17031

2L06

Solution NMR structure of the PBS linker polypeptide domain of phycobilisome linker protein apcE from Synechocystis sp. Northeast Structural Genomics Consortium Target SgR209C

Authors

Ramelot, T.A., Yang, Y., Cort, J.R., Hamilton, K., Ciccosanti, C., Lee, D., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.

Assembly

SLR0335

Entity

1. SLR0335 (polymer, Thiol state: not present), 155 monomers, 17838.98 Da Detail

PQSYFNAAAK RQKYAMKPGL SALEKNAVIK AAYRQIFERD ITKAYSQSIS YLESQVRNGD ISMKEFVRRL AKSPLYRKQF FEPFINSRAL ELAFRHILGR GPSSREEVQK YFSIVSSGGL PALVDALVDS QEYADYFGEE TVPYLRGLEH HHHHH

Formula weight

17838.98 Da

Source organism

Synechocystis sp.

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 90.0 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.0 % (1680 of 1867)	89.9 % (879 of 978)	89.3 % (651 of 729)	93.8 % (150 of 160)
Backbone	92.1 % (844 of 916)	92.3 % (287 of 311)	91.2 % (417 of 457)	94.6 % (140 of 148)
Sidechain	88.8 % (975 of 1098)	88.8 % (592 of 667)	89.0 % (373 of 419)	83.3 % (10 of 12)
Aromatic	66.7 % (132 of 198)	67.7 % (67 of 99)	65.7 % (65 of 99)
Methyl	98.0 % (147 of 150)	97.3 % (73 of 75)	98.7 % (74 of 75)

1. SLR0335

PQSYFNAAAK RQKYAMKPGL SALEKNAVIK AAYRQIFERD ITKAYSQSIS YLESQVRNGD ISMKEFVRRL AKSPLYRKQF FEPFINSRAL ELAFRHILGR GPSSREEVQK YFSIVSSGGL PALVDALVDS QEYADYFGEE TVPYLRGLEH HHHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	20 (±1.0) mM
10	sodium chloride	natural abundance	100 (±5.0) mM
11	calcium chloride	natural abundance	5 (±0.25) mM
12	DTT	natural abundance	100 (±1.0) mM
13	sodium azide	natural abundance	0.02 (±0.001) %
14	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
17	MES	natural abundance	20 (±1.0) mM
18	sodium chloride	natural abundance	100 (±10.0) mM
19	calcium chloride	natural abundance	5 (±0.25) mM
20	DTT	natural abundance	100 (±1.0) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	protein	[U-5% 13C; U-100% 15N]	1.2 (±0.05) mM
23	H2O	natural abundance	95 %
24	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.29 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L06, Strand ID: A Detail

Release date

2010-08-23

Citation

Solution NMR structure of the PBS linker polypeptide domain of phycobilisome linker protein apcE from Synechocystis sp. Northeast Structural Genomics Consortium Target SgR209C
Ramelot, T.A., Yang, Y., Cort, J.R., Hamilton, K., Ciccosanti, C., Lee, D., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T., Kennedy, M.A.
Not known

Related entities 1. SLR0335, : 1 : 2 : 79 entities Detail

Interaction partners 1. SLR0335, : 10 interactors Detail

More details for 10 interactors identified by 3 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

2 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

3 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

4 3D 1H-13C NOESY aliph

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

5 4D 1H-13C NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	20 (±1.0) mM
10	sodium chloride	natural abundance	100 (±5.0) mM
11	calcium chloride	natural abundance	5 (±0.25) mM
12	DTT	natural abundance	100 (±1.0) mM
13	sodium azide	natural abundance	0.02 (±0.001) %
14	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

6 3D HNCO

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

7 3D CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

8 3D HNCACB

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

9 3D (H)CC(CO)NH-TOCSY

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

10 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	20 (±1.0) mM
10	sodium chloride	natural abundance	100 (±5.0) mM
11	calcium chloride	natural abundance	5 (±0.25) mM
12	DTT	natural abundance	100 (±1.0) mM
13	sodium azide	natural abundance	0.02 (±0.001) %
14	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

11 3D HCCH-COSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	20 (±1.0) mM
10	sodium chloride	natural abundance	100 (±5.0) mM
11	calcium chloride	natural abundance	5 (±0.25) mM
12	DTT	natural abundance	100 (±1.0) mM
13	sodium azide	natural abundance	0.02 (±0.001) %
14	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

12 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	20 (±1.0) mM
10	sodium chloride	natural abundance	100 (±5.0) mM
11	calcium chloride	natural abundance	5 (±0.25) mM
12	DTT	natural abundance	100 (±1.0) mM
13	sodium azide	natural abundance	0.02 (±0.001) %
14	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

13 2D 1H-13C HSQC

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
17	MES	natural abundance	20 (±1.0) mM
18	sodium chloride	natural abundance	100 (±10.0) mM
19	calcium chloride	natural abundance	5 (±0.25) mM
20	DTT	natural abundance	100 (±1.0) mM
21	sodium azide	natural abundance	0.02 (±0.001) %
22	protein	[U-5% 13C; U-100% 15N]	1.2 (±0.05) mM
23	H2O	natural abundance	95 %
24	D2O	natural abundance	5 %

14 3D H(CC)(CO)NH-TOCSY

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

15 3D 1H-13C NOESY arom

Instrument

Bruker AvanceIII - 850 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

16 (H)CCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
9	MES	natural abundance	20 (±1.0) mM
10	sodium chloride	natural abundance	100 (±5.0) mM
11	calcium chloride	natural abundance	5 (±0.25) mM
12	DTT	natural abundance	100 (±1.0) mM
13	sodium azide	natural abundance	0.02 (±0.001) %
14	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

17 3D HNCA

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

18 3D HN(CO)CA

Instrument

Varian INOVA - 500 MHz at PNNL

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 (±.5) K, pH 6.5 (±.1)

#	Name	Isotope labeling	Concentration
1	MES	natural abundance	20 (±1.0) mM
2	sodium chloride	natural abundance	100 (±10.0) mM
3	calcium chloride	natural abundance	5 (±0.25) mM
4	DTT	natural abundance	100 (±1.0) mM
5	sodium azide	natural abundance	0.02 (±0.001) %
6	protein	[U-100% 13C; U-100% 15N]	1.4 (±0.05) mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_17031_2l06.nef
Input source #2: Coordindates	2l06.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
PQSYFNAAAKRQKYAMKPGLSALEKNAVIKAAYRQIFERDITKAYSQSISYLESQVRNGDISMKEFVRRLAKSPLYRKQFFEPFINSRALELAFRHILGR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
PQSYFNAAAKRQKYAMKPGLSALEKNAVIKAAYRQIFERDITKAYSQSISYLESQVRNGDISMKEFVRRLAKSPLYRKQFFEPFINSRALELAFRHILGR

-------110-------120-------130-------140-------150-----
GPSSREEVQKYFSIVSSGGLPALVDALVDSQEYADYFGEETVPYLRGLEHHHHHH
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
GPSSREEVQKYFSIVSSGGLPALVDALVDSQEYADYFGEETVPYLRGLEHHHHHH

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	155	0	0	100.0

Content subtype: combined_17031_2l06.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1683		93.5 (chain: A, length: 155)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	2446 (1)	noe	91.0 (chain: A, length: 155)
2	Distance restraints	CNS/XPLOR_distance_constraints_4	OK	116 (1)	hbond	58.1 (chain: A, length: 155)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	196 (196)	.	73.5 (chain: A, length: 155)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 155, Sequence coverage: 93.5%
- Total number of assignments
  - ¹H chemical shifts: 877
  - ¹³C chemical shifts: 650
  - ¹⁵N chemical shifts: 156
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
6	ASN	CG	176.9
12	GLN	CD	180.1
26	ASN	CG	175.7
47	GLN	CD	180.3
55	GLN	CD	179.0
58	ASN	CG	176.9
73	SER	HG	6.24
79	GLN	CD	179.2
86	ASN	CG	175.3
109	GLN	CD	180.2
130	SER	HG	5.64
131	GLN	CD	180.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	978	875	89.5
¹³C chemical shifts	729	640	87.8
¹⁵N chemical shifts	172	156	90.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	311	286	92.0
¹³C chemical shifts	310	273	88.1
¹⁵N chemical shifts	148	136	91.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	667	589	88.3
¹³C chemical shifts	419	367	87.6
¹⁵N chemical shifts	24	20	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	77	75	97.4
¹³C chemical shifts	77	76	98.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	99	63	63.6
¹³C chemical shifts	99	61	61.6

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 155, Sequence coverage: 91.0%
- Total number of restraints: 2446
- Weight of restraints: 1.0 (2446)
- Potential type of restraints: square-well-parabolic (2446)
- Range of distance target values: 2.14, 4.38 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_4
  - Status: OK
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 155, Sequence coverage: 58.1%
  - Total number of restraints: 116
  - Weight of restraints: 1.0 (116)
  - Potential type of restraints: square-well-parabolic (116)
  - Range of distance target values: 2.0, 3.0 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 155, Sequence coverage: 73.5%
- Total number of restraints: 196
- Total number of restraints per polymer type: 196
- Weight of restraints: 1.0 (196)
- Potential type of restraints: square-well-parabolic (196)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords alpha

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BMRB: 17031

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SLR0335, : 1 : 2 : 79 entities Detail

Interaction partners 1. SLR0335, : 10 interactors Detail

Experiments performed 18 experiments Detail

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NMR combined restraints 5 contents Detail