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BMRB: 17033

2L08

Solution NMR Structure of Nonsense mRNA reducing factor 3A from H. Sapiens, Northeast Structural Genomics Consortium Target HR4714B

Authors

Mani, R., Mao, L., Ciccosanti, C., Shastry, R., Acton, T.B., Xiao, R., Swapna, G., Everett, J.K., Montelione, G.B.

Assembly

Nonsense mRNA reducing factor

Entity

1. Nonsense mRNA reducing factor (polymer, Thiol state: not present), 97 monomers, 11577.02 Da Detail

MGHHHHHHSH MVVIRRLPPG LTKEQLEEQL RPLPAHDYFE FFAADLSLYP HLYSRAYINF RNPDDILLFR DRFDGYIFLD SKGLEYPAVV EFAPFQK

Formula weight

11577.02 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.6 %, Completeness: 72.4 %, Completeness (bb): 68.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	72.4 % (873 of 1205)	78.7 % (494 of 628)	62.5 % (302 of 483)	81.9 % (77 of 94)
Backbone	68.9 % (390 of 566)	81.6 % (155 of 190)	56.8 % (163 of 287)	80.9 % (72 of 89)
Sidechain	76.4 % (559 of 732)	77.4 % (339 of 438)	74.4 % (215 of 289)	100.0 % (5 of 5)
Aromatic	52.3 % (91 of 174)	51.7 % (45 of 87)	52.9 % (46 of 87)
Methyl	89.4 % (84 of 94)	91.5 % (43 of 47)	87.2 % (41 of 47)

1. entity

MGHHHHHHSH MVVIRRLPPG LTKEQLEEQL RPLPAHDYFE FFAADLSLYP HLYSRAYINF RNPDDILLFR DRFDGYIFLD SKGLEYPAVV EFAPFQK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5

#	Name	Isotope labeling	Concentration
1	HR4714B	[U-100% 13C; U-100% 15N]	0.82 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	NaCl	natural abundance	100 mM
5	DTT	natural abundance	5 mM
6	NaN3	natural abundance	0.02 %
7	Tris-HCl	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl pH 7.5

#	Name	Isotope labeling	Concentration
8	HR4714B	[U-10% 13C; U-99% 15N]	0.9 mM
9	H2O	natural abundance	90 %
10	D2O	natural abundance	10 %
11	NaCl	natural abundance	100 mM
12	DTT	natural abundance	5 mM
13	NaN3	natural abundance	0.02 %
14	Tris-HCl	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L08, Strand ID: A Detail

Release date

2010-07-08

Citation

olution NMR Structure of Nonsense mRNA reducing factor 3A from H. Sapiens, Northeast Structural Genomics Consortium Target HR4714B
Mani, R., Mao, L., Ciccosanti, C., Shastry, R., Acton, T.B., Xiao, R., Swapna, G., Everett, J., Montelione, G.
Not known

Related entities 1. Nonsense mRNA reducing factor, : 1 : 2 : 26 entities Detail

Interaction partners 1. Nonsense mRNA reducing factor, : 23 interactors Detail

More details for 23 interactors identified by 9 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC