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BMRB: 17044

2L0I

Solution structure of Rtt103 CTD-interacting domain bound to a Ser2 phosphorylated CTD peptide

Authors

Lunde, B.M., Reichow, S.L., Kim, M., Suh, H., Leeper, T.C., Yang, F., Mutschler, H., Buratowski, S., Meinhart, A., Varani, G.

Assembly

Rtt103 CTD complex

Entity

1. Rtt103 (polymer, Thiol state: not present), 142 monomers, 16539.85 Da Detail

MAFSSEQFTT KLNTLEDSQE SISSASKWLL LQYRDAPKVA EMWKEYMLRP SVNTRRKLLG LYLMNHVVQQ AKGQKIIQFQ DSFGKVAAEV LGRINQEFPR DLKKKLSRVV NILKERNIFS KQVVNDIERS LAAALEHHHH HH

2. CTD (polymer, Thiol state: not present), 14 monomers, 1457.495 Da Detail

YSPTSPSYSP TSPS

Total weight

17997.344 Da

Max. entity weight

16539.85 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.2 %, Completeness: 75.5 %, Completeness (bb): 73.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.5 % (1431 of 1895)	84.7 % (843 of 995)	61.1 % (447 of 732)	83.9 % (141 of 168)
Backbone	73.3 % (676 of 922)	86.1 % (266 of 309)	61.4 % (285 of 464)	83.9 % (125 of 149)
Sidechain	78.0 % (878 of 1125)	84.1 % (577 of 686)	67.9 % (285 of 420)	84.2 % (16 of 19)
Aromatic	37.5 % (57 of 152)	71.1 % (54 of 76)	1.4 % (1 of 74)	100.0 % (2 of 2)
Methyl	96.3 % (158 of 164)	97.6 % (80 of 82)	95.1 % (78 of 82)

1. Rtt103

MAFSSEQFTT KLNTLEDSQE SISSASKWLL LQYRDAPKVA EMWKEYMLRP SVNTRRKLLG LYLMNHVVQQ AKGQKIIQFQ DSFGKVAAEV LGRINQEFPR DLKKKLSRVV NILKERNIFS KQVVNDIERS LAAALEHHHH HH

2. CTD

YSPTSPSYSP TSPS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Rtt103	[U-98% 13C; U-98% 15N]	1 mM
2	TRIS	[U-99% 2H]	10 mM
3	sodium chloride	natural abundance	120 mM
4	CTD	natural abundance	1.5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Release date

2010-09-23

Citation

Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain
Lunde, B.M., Reichow, S.L., Kim, M., Suh, H., Leeper, T.C., Yang, F., Mutschler, H., Buratowski, S., Meinhart, A., Varani, G.
Nat. Struct. Mol. Biol. (2010), 17, 1195-1201, PubMed 20818393 , DOI 10.1038/nsmb.1893 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 16411 released on 2010-09-16
    Title Solution structure of Rtt103 CTD interacting domain
  BMRB: 16412 released on 2010-09-16
    Title Solution structure of Rtt103 bound to CTD peptide

Related entities 1. Rtt103, : 1 : 43 entities Detail

Related entities 2. CTD, : 1949 : 750 : 175 : 213 entities Detail

Interaction partners 2. CTD, : 94 interactors Detail

More details for 94 interactors identified by 25 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC