BMRBj - BMREntryMaster

Found 1 Document (0.868 seconds)

BMRB: 17045

2LPE

KSR1 CA1 domain

Authors

Koveal, D., Pinheiro, A., Peti, W., Page, R.

Assembly

KSR1 CA1 monomer

Entity

1. KSR1 CA1 monomer (polymer, Thiol state: all free), 149 monomers, 16699.97 Da Detail

GHMDGGAGAA VSRALQQCGQ LQKLIDISIG SLRGLRTKCS VSNDLTQQEI RTLEAKLVKY ICKQQQSKLS VTPSDRTAEL NSYPRFSDWL YIFNVRPEVV QEIPQELTLD ALLEMDEAKA KEMLRRWGAS TEECSRLQQA LTCLRKVTG

Formula weight

16699.97 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

CNS water refinement using RECOORD scripts

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 96.2 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.2 % (1667 of 1733)	97.1 % (882 of 908)	94.4 % (625 of 662)	98.2 % (160 of 163)
Backbone	97.6 % (865 of 886)	97.4 % (295 of 303)	97.7 % (428 of 438)	97.9 % (142 of 145)
Sidechain	95.2 % (940 of 987)	97.0 % (587 of 605)	92.0 % (335 of 364)	100.0 % (18 of 18)
Aromatic	59.7 % (43 of 72)	91.7 % (33 of 36)	23.5 % (8 of 34)	100.0 % (2 of 2)
Methyl	98.3 % (173 of 176)	97.7 % (86 of 88)	98.9 % (87 of 88)

1. KSR1 CA1

GHMDGGAGAA VSRALQQCGQ LQKLIDISIG SLRGLRTKCS VSNDLTQQEI RTLEAKLVKY ICKQQQSKLS VTPSDRTAEL NSYPRFSDWL YIFNVRPEVV QEIPQELTLD ALLEMDEAKA KEMLRRWGAS TEECSRLQQA LTCLRKVTG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	KSR1 CA1	natural abundance	2 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	100 mM
8	TCEP	natural abundance	0.5 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	KSR1 CA1	[U-99% 15N]	2 mM
10	sodium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	100 mM
12	TCEP	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.44 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LPE, Strand ID: A Detail

Release date

2010-09-07

Citation

Backbone and side chain 1H, 15N and 13C assignments of the KSR1 CA1 domain
Koveal, D., Pinheiro, A.S., Peti, W., Page, R.
Biomol. NMR Assign. (2011), 5, 39-41, PubMed 20737253 , DOI 10.1007/s12104-010-9262-5 , Abstract

Related entities 1. KSR1 CA1 monomer, : 1 : 2 : 12 entities Detail

Interaction partners 1. KSR1 CA1 monomer, : 15 interactors Detail

More details for 15 interactors identified by 7 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

3 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

5 3D (H)CC(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

6 3D HBHA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

7 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
9	KSR1 CA1	[U-99% 15N]	2 mM
10	sodium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	100 mM
12	TCEP	natural abundance	0.5 mM

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

11 2D 1H-1H TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	KSR1 CA1	natural abundance	2 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	100 mM
8	TCEP	natural abundance	0.5 mM

12 2D 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	KSR1 CA1	natural abundance	2 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	100 mM
8	TCEP	natural abundance	0.5 mM

13 2D 1H-1H COSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	KSR1 CA1	natural abundance	2 mM
6	sodium phosphate	natural abundance	20 mM
7	sodium chloride	natural abundance	100 mM
8	TCEP	natural abundance	0.5 mM

14 3D HN(CA)CO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KSR1 CA1	[U-99% 13C; U-99% 15N]	2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	TCEP	natural abundance	0.5 mM

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Found 1 Document (0.868 seconds)

BMRB: 17045

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. KSR1 CA1 monomer, : 1 : 2 : 12 entities Detail

Interaction partners 1. KSR1 CA1 monomer, : 15 interactors Detail

Experiments performed 14 experiments Detail

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