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BMRB: 17050

2XK0

Tudor domain from Drosophila Polycomblike

Authors

Friberg, A., Oddone, A., Klymenko, T., Mueller, J., Sattler, M.

Assembly

Solution structure of the Tudor domain from Drosophila Polycomblike (Pcl)

Entity

1. Solution structure of the Tudor domain from Drosophila Polycomblike (Pcl) (polymer, Thiol state: all free), 69 monomers, 7582.384 Da Detail

GAMAPPVAAP SPAVTYALQE DVFIKCNDGR FYLGTIIDQT SDQYLIRFDD QSEQWCEPDK LRKLGGGSS

Formula weight

7582.384 Da

Source organism

Drosophila melanogaster

Exptl. method

Refine. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.0 %, Completeness (bb): 81.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.0 % (664 of 781)	90.9 % (369 of 406)	74.7 % (227 of 304)	95.8 % (68 of 71)
Backbone	81.7 % (330 of 404)	93.5 % (130 of 139)	68.2 % (137 of 201)	98.4 % (63 of 64)
Sidechain	90.0 % (396 of 440)	89.5 % (239 of 267)	91.6 % (152 of 166)	71.4 % (5 of 7)
Aromatic	63.6 % (42 of 66)	63.6 % (21 of 33)	62.5 % (20 of 32)	100.0 % (1 of 1)
Methyl	95.5 % (63 of 66)	93.9 % (31 of 33)	97.0 % (32 of 33)

1. POLYCOMB PROTEIN PCL

GAMAPPVAAP SPAVTYALQE DVFIKCNDGR FYLGTIIDQT SDQYLIRFDD QSEQWCEPDK LRKLGGGSS

Show sample condition

Sample

Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2XK0, Strand ID: A Detail

Release date

2010-08-11

Citation

Structure of an atypical Tudor domain in the Drosophila Polycomblike protein
Friberg, A., Oddone, A., Klymenko, T., Muller, J., Sattler, M.
Protein Sci. (2010), 19, 1906-1916, PubMed 20669242 , DOI 10.1002/pro.476 , Abstract

Related entities 1. Solution structure of the Tudor domain from Drosophila Polycomblike (Pcl), : 1 : 2 : 3 entities Detail

Interaction partners 1. Solution structure of the Tudor domain from Drosophila Polycomblike (Pcl), : 8 interactors Detail

More details for 8 interactors identified by 3 citations

Experiments performed 10 experiments Detail

1 HNCA

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

2 HNCACB

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

3 CBCA(CO)NH

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

4 (H)CC(CO)NH-TOCSY

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

5 H(CC)(CO)NH-TOCSY

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

6 H(C)CH-TOCSY

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

7 (HB)CB(CGCD)HD

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

8 (HB)CB(CGCDCE)HE

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

9 1H-15N HSQC-NOESY

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

10 1H-13C HMQC-NOESY

Instrument

Bruker DMX - na MHz

Sample

State isotropic, Solvent system 90% water/10% D2O, Pressure 1.000 atm, Temperature 298.500 K, pH 6.300, Details 0.25mM

#	Name	Isotope labeling	Type	Concentration
1	POLYCOMB PROTEIN PCL	[U-13C; U-15N]		0.25 ~ 1.0 mM
2	salt	natural abundance		25 mM
3	buffer	natural abundance		20 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

nullKeywords aromatic cage, methyllysine, NMR, Pcl, Polycomblike, posttranslational modification, PRC2, sDMA, transcriptional regulation, Tudor, POSTTRANSLATIONA MODIFICATION, TRANSCRIPTION