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BMRB: 17073

2L14

Structure of CBP nuclear coactivator binding domain in complex with p53 TAD

Authors

Lee, C., Martinez-Yamout, M.A., Dyson, H.J., Wright, P.E.

Assembly

CBP in complex with p53 TAD

Entity

1. CBP (polymer, Thiol state: not present), 59 monomers, 6561.503 Da Detail

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

2. TAD (polymer, Thiol state: not present), 49 monomers, 5573.085 Da Detail

PLSQETFSDL WKLLPENNVL SPLPSQAMDD LMLSPDDIEQ WFTEDPGPD

Total weight

12134.588 Da

Max. entity weight

6561.503 Da

Source organism

Mus musculus , Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (1249 of 1289)	98.4 % (672 of 683)	94.1 % (461 of 490)	100.0 % (116 of 116)
Backbone	96.8 % (602 of 622)	100.0 % (205 of 205)	93.8 % (302 of 322)	100.0 % (95 of 95)
Sidechain	97.3 % (752 of 773)	97.7 % (467 of 478)	96.4 % (264 of 274)	100.0 % (21 of 21)
Aromatic	74.2 % (46 of 62)	74.2 % (23 of 31)	72.4 % (21 of 29)	100.0 % (2 of 2)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. CBP

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

2. TAD

PLSQETFSDL WKLLPENNVL SPLPSQAMDD LMLSPDDIEQ WFTEDPGPD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CBP	[U-99% 15N]	0.5 mM
2	TAD	[U-99% 15N]	0.5 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
5	CBP	[U-99% 13C; U-99% 15N]	0.5 mM
6	TAD	[U-99% 13C; U-99% 15N]	0.5 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.77 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L14, Strand ID: A, B Detail

Release date

2010-10-21

Citation

Structure of the p53 transactivation domain in complex with the nuclear receptor coactivator binding domain of CREB binding protein
Lee, C., Martinez-Yamout, M.A., Dyson, H., Wright, P.E.
Biochemistry (2010), 49, 9964-9971, PubMed 20961098 , DOI 10.1021/bi1012996 , Abstract

Related entities 1. CBP, : 1 : 3 : 8 : 22 entities Detail

Related entities 2. TAD, : 3 : 24 entities Detail

Interaction partners 1. CBP, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Interaction partners 2. TAD, : 376 : 1 interactors Detail

More details for 377 interactors identified by 182 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC