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An arsenate reductase
Authors
Yu, C., Xia, B., Jin, C.
Assembly
SynArsC
Entity
1. SynArsC (polymer, Thiol state: all free), 134 monomers, 14724.58 Da Detail

GSHMKKVMFV CKRNSCRSQM AEGFAKTLGA GKIAVTSCGL ESSRVHPTAI AMMEEVGIDI SGQTSDPIEN FNADDYDVVI SLCGCGVNLP PEWVTQEIFE DWQLEDPDGQ SLEVFRTVRG QVKERVENLI AKIS


Formula weight
14724.58 Da
Source organism
Synechocystis sp. PCC 6803
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.8 %, Completeness: 90.8 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All90.8 % (1371 of 1510)88.1 % (690 of 783)92.8 % (543 of 585)97.2 % (138 of 142)
Backbone96.0 % (762 of 794)94.9 % (260 of 274)96.4 % (377 of 391)96.9 % (125 of 129)
Sidechain86.8 % (728 of 839)84.5 % (430 of 509)89.9 % (285 of 317)100.0 % (13 of 13)
Aromatic57.8 % (52 of 90)60.0 % (27 of 45)53.5 % (23 of 43)100.0 % (2 of 2)
Methyl94.4 % (136 of 144)93.1 % (67 of 72)95.8 % (69 of 72)

1. SynArsC

GSHMKKVMFV CKRNSCRSQM AEGFAKTLGA GKIAVTSCGL ESSRVHPTAI AMMEEVGIDI SGQTSDPIEN FNADDYDVVI SLCGCGVNLP PEWVTQEIFE DWQLEDPDGQ SLEVFRTVRG QVKERVENLI AKIS

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1SynArsC[U-15N]1 mM
2DSSnatural abundance0.01 %
3sodium azidenatural abundance0.01 %
4sodium chloridenatural abundance50 mM
5sodium phosphatenatural abundance50 mM
6DTTnatural abundance40 mM
7D2Onatural abundance10 %
8H2Onatural abundance90 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
9SynArsC[U-13C; U-15N]1 mM
10DSSnatural abundance0.01 %
11sodium azidenatural abundance0.01 %
12sodium chloridenatural abundance50 mM
13sodium phosphatenatural abundance50 mM
14DTTnatural abundance40 mM
15D2Onatural abundance10 %
16H2Onatural abundance90 %

LACS Plot; CA
Referencing offset: -0.03 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.03 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.03 ppm, Outliers: 4 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2MYP, Strand ID: A Detail


Release date
2010-10-21
Citation
1H, 13C and 15N resonance assignments of the arsenate reductase from Synechocystis sp. strain PCC 6803
Yu, C., Xia, B., Jin, C.
Biomol. NMR Assign. (2011), 5, 85-87, PubMed 20960080 , DOI 10.1007/s12104-010-9273-2 ,
Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail
  BMRB: 17076 released on 2010-10-21
    Title An arsenate reductase
  BMRB: 17077 released on 2010-10-21
    Title An arsenate reductase
  Swiss-Prot: P74313 released on 2014-05-14
    Title ARSC_SYNY3 Entity Glutaredoxin arsenate reductase
Related entities 1. SynArsC, : 1 : 3 : 4 : 91 entities Detail
Experiments performed 14 experiments Detail
NMR combined restraints 3 contents Detail