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BMRB: 17077

2MYT

An arsenate reductase

Authors

Yu, C., Xia, B., Jin, C.

Assembly

SynArsC

Entity

1. SynArsC (polymer, Thiol state: all disulfide bound), 134 monomers, 14676.39 Da Detail

GSHMKKVMFV CKRNSSRSQM AEGFAKTLGA GKIAVTSSGL ESSRVHPTAI AMMEEVGIDI SGQTSDPIEN FNADDYDVVI SLCGSGVNLP PEWVTQEIFE DWQLEDPDGQ SLEVFRTVRG QVKERVENLI AKIS

Formula weight

14676.39 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS11:SG	1:CYS83:SG

Source organism

Synechocystis sp. PCC 6803

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 88.6 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (1338 of 1510)	88.3 % (691 of 783)	88.7 % (519 of 585)	90.1 % (128 of 142)
Backbone	91.3 % (725 of 794)	91.6 % (251 of 274)	91.8 % (359 of 391)	89.1 % (115 of 129)
Sidechain	86.7 % (727 of 839)	86.4 % (440 of 509)	86.4 % (274 of 317)	100.0 % (13 of 13)
Aromatic	58.9 % (53 of 90)	71.1 % (32 of 45)	44.2 % (19 of 43)	100.0 % (2 of 2)
Methyl	97.2 % (140 of 144)	97.2 % (70 of 72)	97.2 % (70 of 72)

1. SynArsC

GSHMKKVMFV CKRNSSRSQM AEGFAKTLGA GKIAVTSSGL ESSRVHPTAI AMMEEVGIDI SGQTSDPIEN FNADDYDVVI SLCGSGVNLP PEWVTQEIFE DWQLEDPDGQ SLEVFRTVRG QVKERVENLI AKIS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	SynArsC	[U-15N]	1 mM
2	DSS	natural abundance	0.01 %
3	sodium azide	natural abundance	0.01 %
4	sodium chloride	natural abundance	50 mM
5	TRIS	natural abundance	50 mM
6	D2O	natural abundance	10 %
7	H2O	natural abundance	90 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	SynArsC	[U-13C; U-15N]	1 mM
9	DSS	natural abundance	0.01 %
10	sodium azide	natural abundance	0.01 %
11	sodium chloride	natural abundance	50 mM
12	TRIS	natural abundance	50 mM
13	D2O	natural abundance	10 %
14	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MYT, Strand ID: A Detail

Release date

2010-10-21

Citation

1H, 13C and 15N resonance assignments of the arsenate reductase from Synechocystis sp. strain PCC 6803
Yu, C., Xia, B., Jin, C.
Biomol. NMR Assign. (2011), 5, 85-87, PubMed 20960080 , DOI 10.1007/s12104-010-9273-2 , Abstract

Entries sharing articles BMRB: 2, Swiss-Prot: 1 entries Detail

  BMRB: 17074 released on 2010-10-21
    Title An arsenate reductase
  BMRB: 17076 released on 2010-10-21
    Title An arsenate reductase
  Swiss-Prot: P74313 released on 2014-05-14
    Title ARSC_SYNY3 Entity Glutaredoxin arsenate reductase