Solution NMR structure ov the chromobox protein Cbx7 with H3K27me3
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.2 % (715 of 859) | 89.7 % (408 of 455) | 76.6 % (256 of 334) | 72.9 % (51 of 70) |
Backbone | 76.3 % (313 of 410) | 82.7 % (115 of 139) | 73.3 % (151 of 206) | 72.3 % (47 of 65) |
Sidechain | 85.2 % (439 of 515) | 90.2 % (285 of 316) | 77.3 % (150 of 194) | 80.0 % (4 of 5) |
Aromatic | 83.8 % (62 of 74) | 94.6 % (35 of 37) | 70.6 % (24 of 34) | 100.0 % (3 of 3) |
Methyl | 82.4 % (56 of 68) | 91.2 % (31 of 34) | 73.5 % (25 of 34) |
1. Cbx7
GEQVFAVESI RKKRVRKGKV EYLVKWKGWP PKYSTWEPEE HILDPRLVMA YEEKEE2. H3K27me3
QLATKAARXS APATGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Cbx7 | [U-13C; U-15N] | 0.5 mM | |
2 | H3K27me3 | natural abundance | 2.7 mM | |
3 | sodium phosphate | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 300 mM | |
5 | PMSF | natural abundance | 0.5 mM | |
6 | TCEP | natural abundance | 0.5 mM | |
7 | Benzamidine | natural abundance | 1 mM | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |