BMRB: 17082

Mouse Prion Protein (121-231) with mutation F175A

Authors

Christen, B., Damberger, F.F., Hornemann, S., Wuthrich, K.

Assembly

mouse prion protein with mutation F175A

Entity

1. mouse prion protein with mutation F175A (polymer, Thiol state: all disulfide bound), 114 monomers, 13317.65 Da Detail

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ YSNQNNAVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Formula weight

13317.65 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 98.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	97.1 % (1288 of 1327)	98.6 % (682 of 692)	94.5 % (479 of 507)	99.2 % (127 of 128)
Backbone	98.7 % (669 of 678)	97.0 % (227 of 234)	99.7 % (332 of 333)	99.1 % (110 of 111)
Sidechain	96.0 % (724 of 754)	99.3 % (455 of 458)	90.3 % (252 of 279)	100.0 % (17 of 17)
Aromatic	78.8 % (104 of 132)	98.5 % (65 of 66)	58.5 % (38 of 65)	100.0 % (1 of 1)
Methyl	100.0 % (88 of 88)	100.0 % (44 of 44)	100.0 % (44 of 44)

1. mPrP121-231 F175A

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ YSNQNNAVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAYYDG RRSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.2 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	prion	[U-99% 13C; U-99% 15N]		1.0 mM
2	sodium acetate	[U-2H]		10 mM
3	H2O	natural abundance		90 %
4	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: 0.4 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.4 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L1E, Strand ID: A Detail

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Release date

2012-08-01

Citation

Prion protein mPrP[F175A](121-231): structure and stability in solution
Christen, B., Hornemann, S., Damberger, F.F., Wuthrich, K.
J. Mol. Biol. (2012), 423, 496-502, PubMed 22922482 , DOI 10.1016/j.jmb.2012.08.011 , Abstract

Related entities 1. mouse prion protein with mutation F175A, : 1 : 13 : 142 entities Detail

Interaction partners 1. mouse prion protein with mutation F175A, : 36 interactors Detail

Experiments performed 2 experiments Detail