BMRB: 17086

1H assignments for ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)

Authors

Patel, M.P., Cribb Fabersunne, C.S., Yang, Y., Kaelin, C.B., Barsh, G.S., Millhauser, G.L.

Assembly

ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)

Entity

1. ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y) (polymer, Thiol state: all disulfide bound), 34 monomers, 3670.276 Da Detail

CVATRNSCKP AAAACCDPAA SCYCRFFRSA CYCR

Formula weight

3670.276 Da

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.8 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	87.8 % (159 of 181)	87.8 % (159 of 181)
Backbone	97.0 % (64 of 66)	97.0 % (64 of 66)
Sidechain	82.6 % (95 of 115)	82.6 % (95 of 115)
Aromatic	44.4 % (8 of 18)	44.4 % (8 of 18)
Methyl	100.0 % (11 of 11)	100.0 % (11 of 11)

1. ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)

CVATRNSCKP AAAACCDPAA SCYCRFFRSA CYCR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5, Details samples was brought to pH 5 using NaOD

#	Name	Isotope labeling	Type	Concentration
1	ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y)	natural abundance		1.0 ~ 2.0 mM
2	D2O	natural abundance		10 %
3	acetic acid	[U-100% 2H]		50 mM
4	sodium azide	natural abundance		0.1 w/v
5	TSP	natural abundance		200 uM
6	H2O	natural abundance		90 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L1J, Strand ID: A Detail

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Release date

2010-07-27

Citation

Loop-swapped chimeras of the agouti-related protein and the agouti signaling protein identify contacts required for melanocortin 1 receptor selectivity and antagonism
Patel, M.P., Cribb Fabersunne, C.S., Yang, Y., Kaelin, C.B., Barsh, G.S., Millhauser, G.L.
J. Mol. Biol. (2010), 404, 45-55, PubMed 20831872 , DOI 10.1016/j.jmb.2010.08.054 , Abstract

Related entities 1. ASIP(93-126, P103A, P105A, P111A, Q115Y, S124Y), : 1 : 10 : 36 entities Detail

Experiments performed 3 experiments Detail