BMRB: 17087

Mouse prion protein (121-231) with the mutations Y169A, Y225A, and Y226A.

Authors

Christen, B., Damberger, F.F., Perez, D.R., Hornemann, S., Wuthrich, K.

Assembly

Prion with Y169A, Y225A, Y226A mutation

Entity

1. Prion with Y169A, Y225A, Y226A mutation (polymer, Thiol state: all disulfide bound), 114 monomers, 13117.46 Da Detail

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ ASNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAAADG RRSS

Formula weight

13117.46 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Mus musculus

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.2 %, Completeness (bb): 83.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.2 % (1169 of 1311)	97.8 % (668 of 683)	74.8 % (374 of 500)	99.2 % (127 of 128)
Backbone	83.3 % (565 of 678)	95.7 % (224 of 234)	69.4 % (231 of 333)	99.1 % (110 of 111)
Sidechain	95.9 % (708 of 738)	98.9 % (444 of 449)	90.8 % (247 of 272)	100.0 % (17 of 17)
Aromatic	79.7 % (94 of 118)	98.3 % (58 of 59)	60.3 % (35 of 58)	100.0 % (1 of 1)
Methyl	97.8 % (90 of 92)	97.8 % (45 of 46)	97.8 % (45 of 46)

1. Prion with Y169A, Y225A, Y226A mutation

GSVVGGLGGY MLGSAMSRPM IHFGNDWEDR YYRENMYRYP NQVYYRPVDQ ASNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCVTQY QKESQAAADG RRSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.2 K, pH 4.5

#	Name	Isotope labeling	Type	Concentration
1	prion	[U-99% 13C; U-99% 15N]		1.4 mM
2	sodium acetate	[U-2H]		10 mM
3	sodium azide	natural abundance		0.02 %
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L1K, Strand ID: A Detail

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Release date

2012-08-01

Citation

Temperature-Induced Misfolding in Prion Protein: Evidence of Multiple Partially Disordered States Stabilized by Non-Native Hydrogen Bonds
Christen, B., Damberger, F.F., Perez, D.R., Hornemann, S., Wuthrich, K.
Biochemistry (2017), 56, 833-844, PubMed 28102071 , DOI 10.1021/acs.biochem.6b01042 , Abstract

Related entities 1. Prion with Y169A, Y225A, Y226A mutation, : 1 : 13 : 142 entities Detail

Interaction partners 1. Prion with Y169A, Y225A, Y226A mutation, : 36 interactors Detail

Experiments performed 2 experiments Detail