BMRB: 17091

Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys2His2 zinc finger induces structural rearrangements of typical DNA base determinant positions

Authors

Malgieri, G., Zaccaro, L., Leone, M., Bucci, E., Esposito, S., Baglivo, I., Del Gatto, A., Scandurra, R., Pedone, P.V., Fattorusso, R., Isernia, C.

Assembly

PROTEIN

Entity

1. SUPERMAN (polymer, Thiol state: all other bound), 37 monomers, 4460.103 Da Detail

WPPRSYTCSF CKREFRSAQA LGGHMNVHRR DRARLRL

2. CD (non-polymer), 112.411 Da

Total weight

4572.514 Da

Max. entity weight

4460.103 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.0 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.0 % (228 of 240)	95.0 % (228 of 240)
Backbone	100.0 % (74 of 74)	100.0 % (74 of 74)
Sidechain	92.8 % (154 of 166)	92.8 % (154 of 166)
Aromatic	100.0 % (24 of 24)	100.0 % (24 of 24)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. SUPERMAN

WPPRSYTCSF CKREFRSAQA LGGHMNVHRR DRARLRL

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 301 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	SUPERMAN	natural abundance		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 301 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
4	SUPERMAN	natural abundance		1 mM
5	D2O	natural abundance		100 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2L1O, Strand ID: A Detail

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Release date

2011-05-31

Citation

Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys₂ His₂ zinc finger induces structural rearrangements of typical DNA base determinant positions
Malgieri, G., Zaccaro, L., Leone, M., Bucci, E., Esposito, S., Baglivo, I., Del Gatto, A., Russo, L., Scandurra, R., Pedone, P.V., Fattorusso, R., Isernia, C.
Biopolymers (2011), 95, 801-810, PubMed 21618209 , DOI 10.1002/bip.21680 , Abstract

Related entities 1. SUPERMAN, : 1 : 3 : 138 entities Detail

Interaction partners 1. SUPERMAN, : 10 interactors Detail

Experiments performed 5 experiments Detail