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BMRB: 17092

2L1P

NMR solution structure of the N-terminal domain of DNA-binding protein SATB1 from Homo sapiens: Northeast Structural Genomics Target HR4435B(179-250)

Authors

Swapna, G., Montelione, A.F., Shastry, R., Ciccosanti, C., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T.

Assembly

DNA-binding protein SATB1

Entity

1. DNA-binding protein SATB1 (polymer, Thiol state: not present), 83 monomers, 9679.970 Da Detail

MGHHHHHHSH MLPPEQWSHT TVRNALKDLL KDMNQSSLAK ECPLSQSMIS SIVNSTYYAN VSAAKCQEFG RWYKHFKKTK DMM

Formula weight

9679.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 89.2 %, Completeness: 86.9 %, Completeness (bb): 86.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (863 of 993)	88.1 % (457 of 519)	85.2 % (327 of 384)	87.8 % (79 of 90)
Backbone	86.6 % (426 of 492)	87.9 % (145 of 165)	85.8 % (212 of 247)	86.3 % (69 of 80)
Sidechain	87.5 % (509 of 582)	88.1 % (312 of 354)	85.8 % (187 of 218)	100.0 % (10 of 10)
Aromatic	67.3 % (70 of 104)	69.2 % (36 of 52)	64.0 % (32 of 50)	100.0 % (2 of 2)
Methyl	100.0 % (62 of 62)	100.0 % (31 of 31)	100.0 % (31 of 31)

1. entity

MGHHHHHHSH MLPPEQWSHT TVRNALKDLL KDMNQSSLAK ECPLSQSMIS SIVNSTYYAN VSAAKCQEFG RWYKHFKKTK DMM

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.08mM HR4435B, 10mM DTT, 50uM DSS, 0.02% NaN3, 100mM NaCl, 5mM CaCl2, 20mM MES,protease inhibitors added.

#	Name	Isotope labeling	Concentration
1	HR4435B	[U-100% 13C; U-100% 15N]	1.08 (±0.2) mM
2	DTT	natural abundance	10 mM
3	DSS	natural abundance	50 mM
4	NaN3	natural abundance	0.02 %
5	NaCl	natural abundance	100 mM
6	CaCl2	natural abundance	5 mM
7	MES	natural abundance	20 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L1P, Strand ID: A Detail

Release date

2012-08-01

Citation

NMR solution structure of the N-terminal domain of DNA-binding protein SATB1 from Homo sapiens: Northeast Structural Genomics Target HR4435B(179-250)
Swapna, G., Montelione, A.F., Shastry, R., Ciccosanti, C., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.
Not known

Related entities 1. DNA-binding protein SATB1, : 1 : 7 : 8 entities Detail

Interaction partners 1. DNA-binding protein SATB1, : 32 interactors Detail

More details for 32 interactors identified by 9 citations