BMRB: 17107

NMR STRUCTURE OF HUMAN INSULIN MUTANT GLY-B20-D-ALA, GLY-B23 2 PRO-B28-LYS, LYS-B29-PRO, 20 STRUCTURES

Authors

Wan, Z.L., Hua, Q.X., Huang, K., Hu, S.Q., Philips, N.B., Katsoyannis, J.WHITT., Weiss, M.A.

Assembly

Human Insulin Mutant

Entity

1. Human Insulin Mutant, entity 1 (polymer, Thiol state: all disulfide bound), 21 monomers, 2383.696 Da Detail

GIVEQCCTSI CSLYQLENYC N

2. Human Insulin (polymer, Thiol state: all disulfide bound), 30 monomers, 3457.971 Da Detail

FVNQHLCGSH LVEALYLVCX ERXFFYTKPT

Total weight

5841.667 Da

Max. entity weight

3457.971 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DISTANCE GEOMETRY AND SIMULATED

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.1 %, Completeness: 95.3 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.3 % (282 of 296)	95.3 % (282 of 296)
Backbone	97.0 % (96 of 99)	97.0 % (96 of 99)
Sidechain	91.9 % (181 of 197)	91.9 % (181 of 197)
Aromatic	94.3 % (33 of 35)	94.3 % (33 of 35)
Methyl	92.9 % (26 of 28)	92.9 % (26 of 28)

1. entity 1

GIVEQCCTSI CSLYQLENYC N

2. Human Insulin

FVNQHLCGSH LVEALYLVCX ERXFFYTKPT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		0.5 mM
2	D2O	natural abundance		10 %
3	H2O	natural abundance		90 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L1Y, Strand ID: A, B Detail

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Release date

2012-08-01

Citation

Chiral Protein Engineering and its Application in G Health
Wan, Z.L., Hua, Q.X., Huang, K., Hu, S.Q., Philips, N.B., Whittaker, J., Katsoyannis, P.G., Weiss, M.A.
J. Biol. Chem.

Related entities 1. Human Insulin Mutant, entity 1, : 112 : 13 : 7 : 90 entities Detail

Related entities 2. Human Insulin, : 1 : 1 : 116 : 100 entities Detail

Interaction partners 1. Human Insulin Mutant, entity 1, : 1 : 1 interactors Detail

Interaction partners 2. Human Insulin, : 6 : 1 interactors Detail

Experiments performed 3 experiments Detail