BMRB: 17108

NMR STRUCTURE OF HUMAN INSULIN MUTANT ILE-A2-LEU, VAL-A3-LEU 2 HIS-B10-ASP, PRO-B28-LYS, LYS-B29-PRO, 20 STRUCTURES

Authors

Wan, Z.L., Hua, Q.X., Huang, K., Hu, S.Q., Philips, N.B., Katsoyannis, J.WHITT., Weiss, M.A.

Assembly

Human Insulin Mutant

Entity

1. Human Insulin Mutant, entity 1 (polymer, Thiol state: all disulfide bound), 21 monomers, 2433.758 Da Detail

GLLEQCCHSI CSLYQLENYC N

2. Human Insulin (polymer, Thiol state: all disulfide bound), 30 monomers, 3407.868 Da Detail

FVNQHLCGSD LVEALYLVCG ERGFFYTKPT

Total weight

5841.626 Da

Max. entity weight

3407.868 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DISTANCE GEOMETRY AND SIMULATED

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.8 %, Completeness (bb): 94.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.8 % (285 of 304)	93.8 % (285 of 304)
Backbone	94.3 % (99 of 105)	94.3 % (99 of 105)
Sidechain	93.5 % (186 of 199)	93.5 % (186 of 199)
Aromatic	85.7 % (30 of 35)	85.7 % (30 of 35)
Methyl	96.3 % (26 of 27)	96.3 % (26 of 27)

1. entity 1

GLLEQCCHSI CSLYQLENYC N

2. Human Insulin

FVNQHLCGSD LVEALYLVCG ERGFFYTKPT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		0.5 mM
2	D2O	natural abundance		10 %
3	H2O	natural abundance		90 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L1Z, Strand ID: A, B Detail

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Release date

2012-08-01

Citation

Chiral Protein Engineering and its Application in G Health
Wan, Z.L., Hua, Q.X., Huang, K., Hu, S.Q., Philips, N.B., Whittaker, J., Katsoyannis, P.G., Weiss, M.A.
J. Biol. Chem.

Related entities 1. Human Insulin Mutant, entity 1, : 1 : 1 : 5 : 213 entities Detail

Related entities 2. Human Insulin, : 1 : 10 : 1 : 115 : 92 entities Detail

Interaction partners 1. Human Insulin Mutant, entity 1, : 4 : 1 interactors Detail

Interaction partners 2. Human Insulin, : 6 : 1 interactors Detail

Experiments performed 3 experiments Detail