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BMRB: 17110

2L21

chicken IGF2R domain 11

Authors

Williams, C., Hoppe, H., Strickland, M., Frago, S., Ellis, R.Z., Wattana-Amorn, P., Prince, S.N., Zaccheo, O.J., Forbes, B., Jones, Y.E., Rezgui, D.Z., Crump, M.P., Hassan, B.A.

Assembly

IGF2R

Entity

1. IGF2R (polymer, Thiol state: all disulfide bound), 154 monomers, 17210.98 Da Detail

MKSNVQNDCR VTNPATGHLF DLTSLKRESG YTITDSHNRK IELNVCAEAK SSCANGAAVC ITDGPKTLNA GKLSKTLTYE DQVLKLVYED GDPCPTDLKM KHKSYFSFVC KSDAGDDSQP VFLSFDEQTC TSYFSWHTSL ACEEEVPRHH HHHH

Formula weight

17210.98 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS46:SG
2	disulfide	sing	1:CYS110:SG	1:CYS142:SG
3	disulfide	sing	1:CYS53:SG	1:CYS60:SG
4	disulfide	sing	1:CYS94:SG	1:CYS130:SG

Source organism

Gallus gallus

Exptl. method

solution NMR

Refine. method

simulated annealing, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 90.2 %, Completeness (bb): 94.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.2 % (1563 of 1733)	90.9 % (813 of 894)	88.1 % (598 of 679)	95.0 % (152 of 160)
Backbone	94.2 % (859 of 912)	95.5 % (295 of 309)	93.2 % (424 of 455)	94.6 % (140 of 148)
Sidechain	87.1 % (843 of 968)	88.5 % (518 of 585)	84.4 % (313 of 371)	100.0 % (12 of 12)
Aromatic	62.5 % (95 of 152)	71.1 % (54 of 76)	53.3 % (40 of 75)	100.0 % (1 of 1)
Methyl	99.3 % (139 of 140)	100.0 % (70 of 70)	98.6 % (69 of 70)

1. IGF2R

MKSNVQNDCR VTNPATGHLF DLTSLKRESG YTITDSHNRK IELNVCAEAK SSCANGAAVC ITDGPKTLNA GKLSKTLTYE DQVLKLVYED GDPCPTDLKM KHKSYFSFVC KSDAGDDSQP VFLSFDEQTC TSYFSWHTSL ACEEEVPRHH HHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	5 (±0.1) %
2	sodium acetate	natural abundance	20 (±0.1) mM
3	EDTA	natural abundance	0.1 (±0.01) mM
4	sodium azide	natural abundance	100 (±0.1) uM
5	IGF2R	[U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
6	H2O	natural abundance	95 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 5.5

#	Name	Isotope labeling	Concentration
7	D2O	natural abundance	5 (±0.1) %
8	sodium phosphate	natural abundance	20 (±0.1) mM
9	EDTA	natural abundance	0.1 (±0.01) mM
10	sodium azide	natural abundance	100 (±0.1) uM
11	IGF2R	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
12	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	7.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	7.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	7.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	7.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.73 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L21, Strand ID: A Detail

Release date

2012-08-29

Citation

An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution
Williams, C., Hoppe, H., Rezgui, D., Strickland, M., Frago, S., Ellis, R.Z., Wattana-Amorn, P., Prince, S.N., Zaccheo, O.J., Forbes, B.E., Jones, E.YVONNE., Crump, M.P., Hassan, A.BASSIM., Grutzner, F., Nolan, C.M., Mungall, A.J.
Science (2012), 338, 1209-1213, PubMed 23197533 , DOI 10.1126/science.1228633 , Abstract

Related entities 1. IGF2R, : 1 : 128 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC