BMRBj - BMREntryMaster

Found 1 Document (0.974 seconds)

BMRB: 17124

2L25

NP_888769.1

Authors

Wahab, A., Serrano, P., Geralt, M., Wilson, I., Wuthrich, K.

Assembly

NP 888769.1

Entity

1. NP 888769.1 (polymer, Thiol state: not present), 141 monomers, 15245.09 Da Detail

GMSQDLIRAA FEKRLSDWAK ARTPALPVAW QNTKFTPPAA GVYLRAYVMP AATISRDAAG DHRQYRGVFQ VNVVMPIGDG SRSAEQVAAE LDALFPVNLV MQSGGLAVRV RTPISNGQPT TGDADHTVPI SLGYDVQFYP E

Formula weight

15245.09 Da

Source organism

Bordetella bronchiseptica

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.4 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.4 % (1488 of 1576)	95.7 % (777 of 812)	91.6 % (568 of 620)	99.3 % (143 of 144)
Backbone	94.7 % (780 of 824)	98.9 % (279 of 282)	90.3 % (372 of 412)	99.2 % (129 of 130)
Sidechain	94.9 % (837 of 882)	94.0 % (498 of 530)	96.2 % (325 of 338)	100.0 % (14 of 14)
Aromatic	86.1 % (105 of 122)	86.9 % (53 of 61)	84.7 % (50 of 59)	100.0 % (2 of 2)
Methyl	98.8 % (164 of 166)	98.8 % (82 of 83)	98.8 % (82 of 83)

1. NP 888769.1

GMSQDLIRAA FEKRLSDWAK ARTPALPVAW QNTKFTPPAA GVYLRAYVMP AATISRDAAG DHRQYRGVFQ VNVVMPIGDG SRSAEQVAAE LDALFPVNLV MQSGGLAVRV RTPISNGQPT TGDADHTVPI SLGYDVQFYP E

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	NP_888769.1	[U-98% 13C; U-98% 15N]	1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	4.5 mM
5	D2O	natural abundance	5 %
6	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L25, Strand ID: A Detail

Release date

2011-05-03

Citation

NMR structure of the Bordetella bronchiseptica protein NP_888769.1 establishes a new phage-related protein family PF13554
Wahab, A., Serrano, P., Geralt, M., Wuthrich, K.
Protein Sci. (2011), 20, 1137-1144, PubMed 21520320 , DOI 10.1002/pro.641 , Abstract

Related entities 1. NP 888769.1, : 1 : 2 entities Detail

Experiments performed 7 experiments Detail

1 2D 15N-1H HSQC