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BMRB: 17127

2L29

COMPLEX STRUCTURE OF E4 mutant HUMAN IGF2R DOMAIN 11 BOUND TO IGF-II

Authors

Williams, C., Hoppe, H., Rezgui, D., Strickland, M., Frago, S., Ellis, R.Z., Wattana-Amorn, P., Prince, S.N., Zaccheo, O.J., Forbes, B., Jones, E.YVONNE., Crump, M.P., Hassan, A.BASSIM.

Assembly

IGF-II in complex with the mutant form of human domain 11

Entity

1. HD11 (polymer, Thiol state: all disulfide bound), 142 monomers, 15417.37 Da Detail

MKSNEHDDCQ VTNPSTGHLF DLSSLSGRAG FTAAYSKSGV VYMSICGENE NCPPGVGACF GQTRISVGKA NKRLRYVDQV LQLVYKDGSP CPSKSGLSYK SVISFVCRPE AGPTNRPMLI SLDKQTCTLF FSWHTPLACE PE

2. IGF2 (polymer, Thiol state: all disulfide bound), 67 monomers, 7475.397 Da Detail

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Total weight

22892.768 Da

Max. entity weight

15417.37 Da

Entity Connection

disulfide 7 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS9:SG	1:CYS46:SG
2	disulfide	sing	1:CYS52:SG	1:CYS59:SG
3	disulfide	sing	1:CYS91:SG	1:CYS127:SG
4	disulfide	sing	1:CYS107:SG	1:CYS139:SG
5	disulfide	sing	2:CYS9:SG	2:CYS47:SG
6	disulfide	sing	2:CYS46:SG	2:CYS51:SG
7	disulfide	sing	2:CYS21:SG	2:CYS60:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 89.5 %, Completeness (bb): 92.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.5 % (2090 of 2336)	90.9 % (1103 of 1213)	86.0 % (786 of 914)	96.2 % (201 of 209)
Backbone	92.7 % (1138 of 1228)	93.4 % (394 of 422)	91.1 % (556 of 610)	95.9 % (188 of 196)
Sidechain	86.2 % (1121 of 1300)	89.1 % (705 of 791)	81.3 % (403 of 496)	100.0 % (13 of 13)
Aromatic	72.9 % (137 of 188)	91.5 % (86 of 94)	53.8 % (50 of 93)	100.0 % (1 of 1)
Methyl	85.9 % (165 of 192)	85.4 % (82 of 96)	86.5 % (83 of 96)

1. HD11

MKSNEHDDCQ VTNPSTGHLF DLSSLSGRAG FTAAYSKSGV VYMSICGENE NCPPGVGACF GQTRISVGKA NKRLRYVDQV LQLVYKDGSP CPSKSGLSYK SVISFVCRPE AGPTNRPMLI SLDKQTCTLF FSWHTPLACE PE

2. IGF2

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
14	IGF2	[U-98% 15N; U-95% 2H]	0.5 ~ 1.0 (±0.05) mM
15	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
16	D2O	natural abundance	5 (±0.1) %
17	sodium azide	natural abundance	100 (±0.1) uM
18	sodium acetate	natural abundance	5 (±0.1) mM
19	EDTA	natural abundance	0.1 (±0.1) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: 0.22 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.658 ppm	internal	indirect	0.2514495
¹H	water	protons	4.658 ppm	internal	direct	1.0
¹⁵N	water	protons	4.658 ppm	internal	indirect	0.1013291

Referencing offset: -0.45 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L29, Strand ID: A, B Detail

Release date

2012-08-29

Citation

An exon splice enhancer primes IGF2:IGF2R binding site structure and function evolution
Williams, C., Hoppe, H., Rezgui, D., Strickland, M., Frago, S., Ellis, R.Z., Wattana-Amorn, P., Prince, S.N., Zaccheo, O.J., Forbes, B.E., Jones, E.YVONNE., Crump, M.P., Hassan, A.BASSIM., Grutzner, F., Nolan, C.M., Mungall, A.J.
Science (2012), 338, 1209-1213, PubMed 23197533 , DOI 10.1126/science.1228633 , Abstract

Related entities 1. HD11, : 1 : 1 : 113 entities Detail

Related entities 2. IGF2, : 1 : 20 entities Detail

Interaction partners 1. HD11, : 39 interactors Detail

More details for 39 interactors identified by 15 citations

Experiments performed 41 experiments Detail

1 3D 1H-13C NOESY

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

2 3D 1H-15N NOESY

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

3 3D 1H-15N NOESY

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

4 3D 1H-13C NOESY

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

5 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz room temp probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
14	IGF2	[U-98% 15N; U-95% 2H]	0.5 ~ 1.0 (±0.05) mM
15	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
16	D2O	natural abundance	5 (±0.1) %
17	sodium azide	natural abundance	100 (±0.1) uM
18	sodium acetate	natural abundance	5 (±0.1) mM
19	EDTA	natural abundance	0.1 (±0.1) mM

6 3D 1H-15N NOESY

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
14	IGF2	[U-98% 15N; U-95% 2H]	0.5 ~ 1.0 (±0.05) mM
15	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
16	D2O	natural abundance	5 (±0.1) %
17	sodium azide	natural abundance	100 (±0.1) uM
18	sodium acetate	natural abundance	5 (±0.1) mM
19	EDTA	natural abundance	0.1 (±0.1) mM

7 2D 1H-15N HSQC

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

8 2D 1H-13C HSQC

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

9 3D HNCO

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

10 3D CBCA(CO)NH

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

11 3D C(CO)NH

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

12 3D HNCA

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

13 3D HNCACB

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

14 3D HNCACB

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

15 3D HCCH-TOCSY

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

16 3D HN(CO)CA

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

17 3D H(CCO)NH

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

18 3D 1H-15N NOESY

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

19 3D 1H-13C NOESY

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

20 2D 1H-15N HSQC

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

21 2D 1H-13C HSQC

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

22 3D CBCA(CO)NH

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

23 3D C(CO)NH

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

24 3D HNCO

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

25 3D HNCA

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

26 3D HNCACB

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

27 3D HN(CO)CA

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

28 3D H(CCO)NH

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

29 3D HCCH-TOCSY

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

30 3D 1H-15N NOESY

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

31 3D 1H-13C NOESY

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

32 2D f2 filter noesy

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

33 2D f2 filter noesy

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

34 2D f2 filter noesy

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

35 2D f2 filter noesy

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

36 2D CN filtered noesy

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

37 2D CN filtered noesy

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

38 2D CN filtered noesy

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

39 2D CN filtered noesy

Instrument

Varian UnityPlus - 900 MHz cryoprobe 900MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

40 2D CN filtered tocsy

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	HD11	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
2	IGF2	natural abundance	1.0 ~ 1.5 (±0.05) mM
3	D2O	natural abundance	5 (±0.1) %
4	sodium azide	natural abundance	100 (±0.1) uM
5	sodium acetate	natural abundance	5 (±0.1) mM
6	EDTA	natural abundance	0.1 (±0.1) mM

41 2D CN filtered tocsy

Instrument

Varian VNMRS - 600 MHz cryoprobe 600MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 4.2 (±0.1)

#	Name	Isotope labeling	Concentration
7	IGF2	[U-98% 13C; U-98% 15N]	0.5 ~ 1.0 (±0.05) mM
8	HD11	natural abundance	1.0 ~ 1.5 (±0.05) mM
9	D2O	natural abundance	5 (±0.1) %
10	sodium azide	natural abundance	100 (±0.1) uM
11	sodium acetate	natural abundance	5 (±0.1) mM
12	EDTA	natural abundance	0.1 (±0.1) mM
13	H2O	natural abundance	95 (±0.1) %

nullKeywords CATION-INDEPENDENT MANNOSE-6-PHOSPHATE, Genomic imprinting, Insulin-like growth factor 2, mannose 6 phosphate receptor, NMR structure, protein evolution, ligand trap, NMR complex structure

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Found 1 Document (3.692 seconds)

BMRB: 17127

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HD11, : 1 : 1 : 113 entities Detail

Related entities 2. IGF2, : 1 : 20 entities Detail

Interaction partners 1. HD11, : 39 interactors Detail

Experiments performed 41 experiments Detail

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