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BMRB: 17132

2L2D

Solution Structure of human UBA-like domain of OTUD7A_11_83, NESG target HT6304a/OCSP target OTUD7A_11_83/SGC-Toronto

Authors

Wu, B., Yee, A., Lemak, A., Gutmanas, A., Houliston, S., Semesi, A., Dhe-Paganon, S., Arrowsmith, C.H.

Assembly

amino-terminal UBA domain of OTUD7A

Entity

1. amino-terminal UBA domain of OTUD7A (polymer, Thiol state: all free), 73 monomers, 8037.855 Da Detail

SAECWAALLH DPMTLDMDAV LSDFVRSTGA EPGLARDLLE GKNWDLTAAL SDYEQLRQVH TANLPHVFNE GRG

Formula weight

8037.855 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.3 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (776 of 806)	96.4 % (397 of 412)	96.2 % (305 of 317)	96.1 % (74 of 77)
Backbone	98.4 % (425 of 432)	97.3 % (144 of 148)	99.5 % (213 of 214)	97.1 % (68 of 70)
Sidechain	94.8 % (419 of 442)	95.8 % (253 of 264)	93.6 % (160 of 171)	85.7 % (6 of 7)
Aromatic	81.3 % (52 of 64)	90.6 % (29 of 32)	70.0 % (21 of 30)	100.0 % (2 of 2)
Methyl	95.3 % (82 of 86)	95.3 % (41 of 43)	95.3 % (41 of 43)

1. OTUD7A 11 83

SAECWAALLH DPMTLDMDAV LSDFVRSTGA EPGLARDLLE GKNWDLTAAL SDYEQLRQVH TANLPHVFNE GRG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	OTUD7A_11_83	[U-100% 13C; U-100% 15N]	0.5 mM
2	TRIS	[U-100% 2H]	10 mM
3	sodium chloride	natural abundance	300 mM
4	zinc sulphate	natural abundance	10 uM
5	DTT	[U-100% 2H]	10 mM
6	benzamidine	natural abundance	10 mM
7	inhibitor cocktail	natural abundance	1 na
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
10	OTUD7A_11_83	[U-10% 13C; U-99% 15N]	0.5 mM
11	TRIS	[U-100% 2H]	10 mM
12	sodium chloride	natural abundance	300 mM
13	zinc sulphate	natural abundance	10 uM
14	DTT	[U-100% 2H]	10 mM
15	benzamidine	natural abundance	10 mM
16	inhibitor cocktail	natural abundance	1 na
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L2D, Strand ID: A Detail

Release date

2010-09-01

Citation

The amino-terminal UBA domain of OTUD7A
Wu, B., Asinas, A., Yee, A., Doherty, R., Lemak, A., Gutmanas, A., Houliston, S., Semesi, A., Arrowsmith, C.H., Dhe-Paganon, S.
Not known

Related entities 1. amino-terminal UBA domain of OTUD7A, : 1 : 2 : 2 : 10 entities Detail

Interaction partners 1. amino-terminal UBA domain of OTUD7A, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 3D HNCO