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BMRB: 17147

2L7F

Chemical Shifts of the Pitx2 Homeodomain

Authors

Doerdelmann, T., Kojetin, D.J., Baird-Titus, J.M., Rance, M.

Assembly

Pitx2 homeodomain

Entity

1. Pitx2 homeodomain (polymer, Thiol state: not present), 68 monomers, 8470.392 Da Detail

GSQRRQRTHF TSQQLQELEA TFQRNRYPDM STREEIAVWT NLTEARVRVW FKNRRAKWRK REEFIVTD

Formula weight

8470.392 Da

Source organism

Exptl. method

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 90.1 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (776 of 861)	86.2 % (395 of 458)	94.1 % (305 of 324)	96.2 % (76 of 79)
Backbone	97.5 % (396 of 406)	97.1 % (132 of 136)	98.0 % (199 of 203)	97.0 % (65 of 67)
Sidechain	85.6 % (447 of 522)	81.7 % (263 of 322)	92.0 % (173 of 188)	91.7 % (11 of 12)
Aromatic	70.5 % (62 of 88)	72.7 % (32 of 44)	65.9 % (27 of 41)	100.0 % (3 of 3)
Methyl	100.0 % (58 of 58)	100.0 % (29 of 29)	100.0 % (29 of 29)

1. Pitx2 homeodomain

GSQRRQRTHF TSQQLQELEA TFQRNRYPDM STREEIAVWT NLTEARVRVW FKNRRAKWRK REEFIVTD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: -0.5 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L7F, Strand ID: P Detail

Release date

2010-11-08

Citation

1H, 13C and 15N chemical shift assignments for the human Pitx2 homeodomain and a R24H homeodomain mutant
Doerdelmann, T., Kojetin, D.J., Baird-Titus, J.M., Rance, M.
Biomol. NMR Assign. (2011), 5, 105-107, PubMed 21052876 , DOI 10.1007/s12104-010-9278-x , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 17145 released on 2010-11-08
Title Chemical Shifts of the R24H Pitx2 Homeodomain Mutant

Related entities 1. Pitx2 homeodomain, : 1 : 1 : 29 : 242 entities Detail

Interaction partners 1. Pitx2 homeodomain, : 14 interactors Detail

More details for 14 interactors identified by 9 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

3 3D HNCACB

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

4 3D HNCA

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

5 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

6 3D HNCO

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

7 3D HBHA(CO)NH

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

8 3D H(CCO)NH

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

10 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 295 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	Pitx2 homeodomain	[U-98% 13C; U-98% 15N]		1.5 mM
2	phosphate buffer	natural abundance		10 mM
3	sodium sulfate	natural abundance		150 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

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