BMRBj - BMREntryMaster

Found 1 Document (0.737 seconds)

BMRB: 17150

2L2Q

Solution Structure of cellobiose-specific phosphotransferase IIB component protein from Borrelia burgdorferi. Seattle Structure Genomics Center for Infectious Disease (SSGCID).

Authors

Yang, F., Barnwal, R., Varani, G.

Assembly

phosphotransferase IIB

Entity

1. phosphotransferase IIB (polymer, Thiol state: all free), 109 monomers, 11966.81 Da Detail

GPGSMNILLV CGAGMSTSML VQRIEKYAKS KNINATIEAI AETRLSEVVD RFDVVLLAPQ SRFNKKRLEE ITKPKGIPIE IINTIDYGTM NGEKVLQLAI NAFNNKSSV

Formula weight

11966.81 Da

Source organism

Borreliella burgdorferi

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 86.2 %, Completeness (bb): 92.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (1097 of 1273)	85.1 % (565 of 664)	85.8 % (422 of 492)	94.0 % (110 of 117)
Backbone	92.6 % (598 of 646)	91.9 % (203 of 221)	92.8 % (297 of 320)	93.3 % (98 of 105)
Sidechain	81.8 % (596 of 729)	81.7 % (362 of 443)	81.0 % (222 of 274)	100.0 % (12 of 12)
Aromatic	47.8 % (22 of 46)	95.7 % (22 of 23)	0.0 % (0 of 23)
Methyl	99.3 % (143 of 144)	98.6 % (71 of 72)	100.0 % (72 of 72)

1. phosphotransferase IIB

GPGSMNILLV CGAGMSTSML VQRIEKYAKS KNINATIEAI AETRLSEVVD RFDVVLLAPQ SRFNKKRLEE ITKPKGIPIE IINTIDYGTM NGEKVLQLAI NAFNNKSSV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	phosphotransferase_IIB	[U-95% 15N]	1.2 mM
2	potassium phosphate	natural abundance	20 mM
3	potassium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	phosphotransferase_IIB	[U-95% 13C; U-95% 15N]	1.2 mM
8	potassium phosphate	natural abundance	20 mM
9	potassium chloride	natural abundance	100 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
13	phosphotransferase_IIB	natural abundance	1.1 mM
14	potassium phosphate	natural abundance	20 mM
15	potassium chloride	natural abundance	100 mM
16	DTT	natural abundance	1 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L2Q, Strand ID: A Detail

Release date

2010-09-15

Citation

Solution Structure of cellobiose-specific phosphotransferase IIB component protein from Borrelia burgdorferi
Yang, F., Barnwal, R., Varani, G.
Not known

Related entities 1. phosphotransferase IIB, : 1 : 16 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC