BMRB: 17152

Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)

Authors

Nolde, S.B., Barinov, N.A., Balashova, T.A., Arseniev, A.S.

Assembly

EcAMP1

Entity

1. EcAMP1 (polymer, Thiol state: all disulfide bound), 37 monomers, 4278.703 Da Detail

GSGRGSCRSQ CMRRHEDEPW RVQECVSQCR RRRGGGD

Formula weight

4278.703 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS29:SG
2	disulfide	sing	1:CYS11:SG	1:CYS25:SG

Source organism

Echinochloa crus-galli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.2 %, Completeness (bb): 98.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.2 % (217 of 221)	98.2 % (217 of 221)
Backbone	98.7 % (78 of 79)	98.7 % (78 of 79)
Sidechain	97.9 % (139 of 142)	97.9 % (139 of 142)
Aromatic	87.5 % (7 of 8)	87.5 % (7 of 8)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. EcAMP1

GSGRGSCRSQ CMRRHEDEPW RVQECVSQCR RRRGGGD

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.62

#	Name	Isotope labeling	Type	Concentration
1	EcAMP1	natural abundance		0.7 mg/mL
2	KOH	natural abundance		10 mM
3	H2O	natural abundance		95 %
4	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2L2R, Strand ID: A Detail

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Release date

2011-05-03

Citation

Disulfide-stabilized helical hairpin structure and activity of a novel antifungal peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)
Nolde, S.B., Vassilevski, A.A., Rogozhin, E.A., Barinov, N.A., Balashova, T.A., Samsonova, O.V., Baranov, Y.V., Feofanov, A.V., Egorov, T.A., Arseniev, A.S., Grishin, E.V.
J. Biol. Chem. (2011), 286, 25145-25153, PubMed 21561864 , DOI 10.1074/jbc.M110.200378 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. EcAMP1, : 1 : 1 : 7 entities Detail

Experiments performed 3 experiments Detail