BMRBj - BMREntryMaster

Found 1 Document (7.464 seconds)

BMRB: 17158

2L31

DNA repair protein zinc finger 2

Authors

Neuhaus, D., Eustermann, S., Yang, J., Videler, H.

Assembly

PARP-1 Finger 2

Entity

1. PARP-1 Finger 2 (polymer, Thiol state: all other bound), 112 monomers, 12570.25 Da Detail

GSKAEKTLGD FAAEYAKSNR STCKGCMEKI EKGQVRLSKK MVDPEKPQLG MIDRWYHPGC FVKNREELGF RPEYSASQLK GFSLLATEDK EALKKQLPGV KSEGKRKGDE VD

2. ZN (non-polymer), 65.409 Da

Total weight

12635.659 Da

Max. entity weight

12570.25 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS23:SG	2:ZN1:ZN
2	na	sing	1:CYS26:SG	2:ZN1:ZN
3	na	sing	1:HIS57:ND1	2:ZN1:ZN
4	na	sing	1:CYS60:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 83.2 %, Completeness (bb): 82.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.2 % (1104 of 1327)	86.6 % (613 of 708)	75.2 % (380 of 505)	97.4 % (111 of 114)
Backbone	82.6 % (547 of 662)	96.5 % (222 of 230)	68.0 % (221 of 325)	97.2 % (104 of 107)
Sidechain	85.9 % (658 of 766)	81.8 % (391 of 478)	92.5 % (260 of 281)	100.0 % (7 of 7)
Aromatic	92.5 % (74 of 80)	92.5 % (37 of 40)	92.3 % (36 of 39)	100.0 % (1 of 1)
Methyl	100.0 % (84 of 84)	100.0 % (42 of 42)	100.0 % (42 of 42)

1. PARP-1 Finger 2

GSKAEKTLGD FAAEYAKSNR STCKGCMEKI EKGQVRLSKK MVDPEKPQLG MIDRWYHPGC FVKNREELGF RPEYSASQLK GFSLLATEDK EALKKQLPGV KSEGKRKGDE VD

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	PARP-1 Finger 2	[U-98% 15N; U-98% 13C]	0.5 ~ 1.0 mM
2	TRIS	[U-99% 2H]	50 mM
3	sodium chloride	natural abundance	200 mM
4	zinc sulfate	natural abundance	150 uM
5	DTT	[U-99% 2H]	4 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	external	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	TSP	methyl protons	external	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 2L31, Strand ID: A Detail

Release date

2011-01-26

Citation

The DNA-binding domain of human PARP-1 interacts with DNA single-strand breaks as a monomer through its second zinc finger
Eustermann, S., Videler, H., Yang, J., Cole, P.T., Gruszka, D., Veprintsev, D., Neuhaus, D.
J. Mol. Biol. (2011), 407, 149-170, PubMed 21262234 , DOI 10.1016/j.jmb.2011.01.034 , Abstract

Related entities 1. PARP-1 Finger 2, : 1 : 4 : 3 : 41 entities Detail

Interaction partners 1. PARP-1 Finger 2, : 182 interactors Detail

More details for 182 interactors identified by 72 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC