BMRBj - BMREntryMaster

Found 1 Document (0.952 seconds)

BMRB: 17160

2LHS

1H, 13C, 15N resonance assignment of the chitin-binding protein CBP21 from Serratia marcescens

Authors

Vaaje-Kolstad, G., Eijsink, V.G. H., Aachmann, F.L.

Assembly

CBP21

Entity

1. CBP21 (polymer, Thiol state: all disulfide bound), 170 monomers, 18793.65 Da Detail

HGYVESPASR AYQCKLQLNT QCGSVQYEPQ SVEGLKGFPQ AGPADGHIAS ADKSTFFELD QQTPTRWNKL NLKTGPNSFT WKLTARHSTT SWRYFITKPN WDASQPLTRA SFDLTPFCQF NDGGAIPAAQ VTHQCNIPAD RSGSHVILAV WDIADTANAF YQAIDVNLSK

Formula weight

18793.65 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS22:SG
2	disulfide	sing	1:CYS118:SG	1:CYS135:SG

Source organism

Serratia marcescens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.5 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.5 % (1706 of 1950)	86.9 % (875 of 1007)	86.1 % (652 of 757)	96.2 % (179 of 186)
Backbone	98.1 % (979 of 998)	97.6 % (331 of 339)	99.2 % (496 of 500)	95.6 % (152 of 159)
Sidechain	79.7 % (886 of 1112)	81.4 % (544 of 668)	75.5 % (315 of 417)	100.0 % (27 of 27)
Aromatic	30.0 % (63 of 210)	50.5 % (53 of 105)	5.0 % (5 of 100)	100.0 % (5 of 5)
Methyl	97.6 % (160 of 164)	97.6 % (80 of 82)	97.6 % (80 of 82)

1. CBP21

HGYVESPASR AYQCKLQLNT QCGSVQYEPQ SVEGLKGFPQ AGPADGHIAS ADKSTFFELD QQTPTRWNKL NLKTGPNSFT WKLTARHSTT SWRYFITKPN WDASQPLTRA SFDLTPFCQF NDGGAIPAAQ VTHQCNIPAD RSGSHVILAV WDIADTANAF YQAIDVNLSK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	CBP21	[U-98% 13C; U-98% 15N]	1.2 ~ 1.4 (±0.1) mM
7	sodium phosphate	natural abundance	20 (±0.1) mM
8	sodium chloride	natural abundance	10 (±0.1) mM
9	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
10	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
11	sodium phosphate	natural abundance	20 (±0.1) mM
12	sodium chloride	natural abundance	10 (±0.1) mM
13	D2O	natural abundance	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
14	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
15	sodium phosphate	natural abundance	20 (±0.1) mM
16	sodium chloride	natural abundance	10 (±0.1) mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.53 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LHS, Strand ID: A Detail

Release date

2010-11-08

Citation

1H, 13C, 15N resonance assignment of the chitin-binding protein CBP21 from Serratia marcescens
Aachmann, F.L., Eijsink, V.G.H., Vaaje-Kolstad, G.
Biomol. NMR Assign. (2011), 5, 117-119, PubMed 21052875 , DOI 10.1007/s12104-010-9281-2 , Abstract

Related entities 1. CBP21, : 1 : 1 : 1 : 57 entities Detail

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	CBP21	[U-98% 13C; U-98% 15N]	1.2 ~ 1.4 (±0.1) mM
7	sodium phosphate	natural abundance	20 (±0.1) mM
8	sodium chloride	natural abundance	10 (±0.1) mM
9	D2O	natural abundance	100 %

3 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
10	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
11	sodium phosphate	natural abundance	20 (±0.1) mM
12	sodium chloride	natural abundance	10 (±0.1) mM
13	D2O	natural abundance	100 %

4 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
14	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
15	sodium phosphate	natural abundance	20 (±0.1) mM
16	sodium chloride	natural abundance	10 (±0.1) mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

5 2D 1H-1H COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
10	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
11	sodium phosphate	natural abundance	20 (±0.1) mM
12	sodium chloride	natural abundance	10 (±0.1) mM
13	D2O	natural abundance	100 %

6 2D 1H-1H COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
14	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
15	sodium phosphate	natural abundance	20 (±0.1) mM
16	sodium chloride	natural abundance	10 (±0.1) mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

7 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
10	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
11	sodium phosphate	natural abundance	20 (±0.1) mM
12	sodium chloride	natural abundance	10 (±0.1) mM
13	D2O	natural abundance	100 %

8 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
14	CBP21	natural abundance	1.2 ~ 1.4 (±0.1) mM
15	sodium phosphate	natural abundance	20 (±0.1) mM
16	sodium chloride	natural abundance	10 (±0.1) mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

9 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

10 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

11 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

12 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

13 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

14 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	CBP21	[U-98% 13C; U-98% 15N]	1.2 ~ 1.4 (±0.1) mM
7	sodium phosphate	natural abundance	20 (±0.1) mM
8	sodium chloride	natural abundance	10 (±0.1) mM
9	D2O	natural abundance	100 %

15 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	CBP21	[U-98% 13C; U-98% 15N]	1.2 ~ 1.4 (±0.1) mM
7	sodium phosphate	natural abundance	20 (±0.1) mM
8	sodium chloride	natural abundance	10 (±0.1) mM
9	D2O	natural abundance	100 %

16 3D HBHANH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

17 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

18 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	CBP21	[U-98% 13C; U-98% 15N]	1.0 ~ 1.6 (±0.1) mM
2	sodium phosphate	natural abundance	20 (±0.1) mM
3	sodium chloride	natural abundance	10 (±0.1) mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

nullKeywords carbohydrate-binding module (CBM), CBM33, Chitin, Chitin-binding protein (CBP)

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Found 1 Document (0.952 seconds)

BMRB: 17160

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CBP21, : 1 : 1 : 1 : 57 entities Detail

Experiments performed 18 experiments Detail

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