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BMRB: 17188

2L3E

Solution structure of P2a-J2a/b-P2b of human telomerase RNA

Authors

Zhang, Q., Kim, N., Peterson, R.D., Wang, Z., Feigon, J.

Assembly

35-MER

Entity

1. 35-MER (polymer, Thiol state: not present), 35 monomers, 11227.61 Da Detail

GGCUUUUGCU CCCCGUGCUU CGGCACGGAA AAGCC

Formula weight

11227.61 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 100.0 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	97.7 % (552 of 565)	96.8 % (302 of 312)	100.0 % (235 of 235)	83.3 % (15 of 18)
Suger, PO4	100.0 % (385 of 385)	100.0 % (210 of 210)	100.0 % (175 of 175)
Nucleobase	92.8 % (167 of 180)	90.2 % (92 of 102)	100.0 % (60 of 60)	83.3 % (15 of 18)
Aromatic	95.5 % (149 of 156)	94.9 % (74 of 78)	100.0 % (60 of 60)	83.3 % (15 of 18)

1. RNA 35-MER

GGCUUUUGCU CCCCGUGCUU CGGCACGGAA AAGCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	RNA_35-MER	natural abundance	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	P2ab RNA (35-MER)	natural abundance		1.0 mM
16	D2O	natural abundance		100 %

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
17	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]		1.0 mM
18	D2O	natural abundance		100 %

Release date

2010-10-25

Citation

Structurally conserved five nucleotide bulge determines the overall topology of the core domain of human telomerase RNA
Zhang, Q., Kim, N., Peterson, R.D., Wang, Z., Feigon, J.
Proc. Natl. Acad. Sci. U. S. A. (2010), 107, 18761-18768, PubMed 20966348 , DOI 10.1073/pnas.1013269107 , Abstract

Related entities 1. 35-MER, : 1 entities Detail

Experiments performed 40 experiments Detail

1 2D 11echo NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	RNA_35-MER	natural abundance	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

2 2D Watergate NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
1	RNA_35-MER	natural abundance	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

3 2D 1H-1H NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	P2ab RNA (35-MER)	natural abundance		1.0 mM
16	D2O	natural abundance		100 %

4 2D 1H-1H TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	P2ab RNA (35-MER)	natural abundance		1.0 mM
16	D2O	natural abundance		100 %

5 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

7 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 2D 13C Filtered/Edited NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

9 2D 13C Filtered/Edited NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

10 2D 13C Filtered/Edited NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

11 2D 13C Filtered/Edited NOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

12 2D HCCH-COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

13 2D HCCH-COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

14 2D HCCH-COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

15 2D HCCH-COSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

16 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

17 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

18 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

19 3D HCCH-TOCSY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

20 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

21 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

22 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

23 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

24 2D 31P Spin echo difference CT-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

25 2D 31P Spin echo difference CT-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

26 2D 31P Spin echo difference CT-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

27 2D 31P Spin echo difference CT-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

28 2D 31P Spin echo difference CT-HCCH correlation

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

29 2D 31P Spin echo difference CT-HCCH correlation

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

30 2D 31P Spin echo difference CT-HCCH correlation

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

31 2D 31P Spin echo difference CT-HCCH correlation

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

32 2D 1H-13C HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
17	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]		1.0 mM
18	D2O	natural abundance		100 %

33 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

34 2D 1H-13C S3CT-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

35 2D 1H-13C S3CT-HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

36 2D HNNOESY

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283.15 K, pH 6.4

#	Name	Isotope labeling	Concentration
4	P2ab RNA (35-MER)	[U-98% 13C; U-98% 15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

37 3D HCNCH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
7	P2ab RNA (35-MER)	[U-13C; U-15N]-Ade		1.0 mM
8	D2O	natural abundance		100 %

38 3D HCNCH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
9	P2ab RNA (35-MER)	[U-13C; U-15N]-Cyt		1.0 mM
10	D2O	natural abundance		100 %

39 3D HCNCH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
11	P2ab RNA (35-MER)	[U-13C; U-15N]-Gua		1.0 mM
12	D2O	natural abundance		100 %

40 3D HCNCH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293.15 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
13	P2ab RNA (35-MER)	[U-13C; U-15N]-Ura		1.0 mM
14	D2O	natural abundance		100 %

NMR combined restraints 6 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_17188_2l3e.nef
Input source #2: Coordindates	2l3e.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30-----
GGCUUUUGCUCCCCGUGCUUCGGCACGGAAAAGCC
|||||||||||||||||||||||||||||||||||
GGCUUUUGCUCCCCGUGCUUCGGCACGGAAAAGCC

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	35	0	0	100.0

Content subtype: combined_17188_2l3e.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	605		100.0 (chain: A, length: 35)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	OK	785 (1)	noe	100.0 (chain: A, length: 35)
2	Distance restraints	CNS/XPLOR_distance_constraints_3	Warning	73 (1)	hbond	80.0 (chain: A, length: 35)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_4	OK	230 (230)	.	100.0 (chain: A, length: 35)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_5	OK	76 (76)	.	85.7 (chain: A, length: 35)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 35, Sequence coverage: 100.0%

--------10--------20--------30-----
GGCUUUUGCUCCCCGUGCUUCGGCACGGAAAAGCC
|||||||||||||||||||||||||||||||||||
GGCUUUUGCUCCCCGUGCUUCGGCACGGAAAAGCC

Total number of assignments
- ¹H chemical shifts: 309
- ¹³C chemical shifts: 235
- ¹⁵N chemical shifts: 61

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
1	G	N9	168.447
2	G	N9	169.341
3	C	N1	151.067
3	C	N4	99.006
4	U	N1	154.289
5	U	N1	154.534
6	U	N1	154.644
7	U	N1	153.608
8	G	N9	169.023
9	C	N1	151.941
10	U	N1	152.386
11	C	N1	151.935
12	C	N1	152.285
13	C	N1	152.242
13	C	N4	98.654
14	C	N1	150.709
14	C	N4	98.342
15	G	H21	8.269
15	G	H22	6.958
15	G	N9	169.095
16	U	N1	153.709
17	G	N9	169.785
18	C	N1	151.217
18	C	N4	99.761
19	U	N1	154.876
20	U	N1	151.877
21	C	N1	150.611
21	C	N4	93.902
22	G	H22	6.665
22	G	N9	171.718
23	G	H21	8.792
23	G	H22	6.426
23	G	N2	75.818
23	G	N9	170.408
24	C	N1	151.182
24	C	N4	97.928
25	A	N9	170.864
26	C	N1	150.404
26	C	N4	98.492
27	G	N9	168.71
28	G	N9	168.565
29	A	H61	7.718
29	A	H62	6.586
29	A	N6	83.625
29	A	N9	169.136
30	A	N9	170.016
31	A	N9	170.31
32	A	N9	170.362
33	G	N9	169.032
34	C	N1	151.227
34	C	N4	99.458
35	C	N1	152.612
35	C	N4	97.691

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	312	302	96.8
¹³C chemical shifts	235	235	100.0
¹⁵N chemical shifts	18	15	83.3

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	210	210	100.0
¹³C chemical shifts	175	175	100.0

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	102	92	90.2
¹³C chemical shifts	60	60	100.0
¹⁵N chemical shifts	18	15	83.3

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	20	20	100.0
¹³C chemical shifts	20	20	100.0

Histogram of normalized assigned chemical shifts

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 35, Sequence coverage: 100.0%
- Total number of restraints: 785
- Weight of restraints: 1.0 (785)
- Potential type of restraints: square-well-parabolic (785)
- Range of distance target values: 2.5, 4.65 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: CNS/XPLOR_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 35, Sequence coverage: 80.0%
  - Total number of restraints: 73
  - Weight of restraints: 1.0 (73)
  - Potential type of restraints: square-well-parabolic (73)
  - Range of distance target values: 2.0, 3.5 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 35, Sequence coverage: 100.0%
- Total number of restraints: 230
- Total number of restraints per polymer type: 230
- Weight of restraints: 1.0 (230)
- Potential type of restraints: square-well-parabolic (230)
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_5
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 35, Sequence coverage: 85.7%
- Total number of restraints: 76
- Potential type of restraints: square-well-parabolic (76)
- Range of observed RDC values: -41.96, 40.56 (Hz)
- Histogram of observed RDC values
- RDC restraints per residue
  - Chain_ID: A
    None

Keywords hTR, human telomerase RNA, J2ab, P2a, P2b

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Found 1 Document (0.416 seconds)

BMRB: 17188

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Related entities 1. 35-MER, : 1 entities Detail

Experiments performed 40 experiments Detail

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