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BMRB: 17191

2L3F

Solution NMR Structure of a putative Uracil DNA glycosylase from Methanosarcina acetivorans, Northeast Structural Genomics Consortium Target MvR76

Authors

Aramini, J.M., Hamilton, K., Ciccosanti, C.T., Wang, H., Lee, H., Rost, B., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T.

Assembly

MvR76

Entity

1. MvR76 (polymer, Thiol state: all free), 166 monomers, 19116.36 Da Detail

MIKRGFPAVL DENTEILILG SLPSDESIRK QQYYGNPGND FWRLVGHAIG ENLQDMAYEK KLKTLKHNRI GLWDVFKAGS REGSQDSKIG DEEINDFSGL KEMVPKLRLI CFNGRKAGEY EPLLRGMGYE TKVLPSSSGA NRRFSKNRES EWEAVFRHLE HHHHHH

Formula weight

19116.36 Da

Source organism

Methanosarcina acetivorans

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

RDCs, assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 92.5 %, Completeness (bb): 95.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.5 % (1837 of 1986)	92.2 % (965 of 1047)	92.0 % (702 of 763)	96.6 % (170 of 176)
Backbone	95.5 % (940 of 984)	95.9 % (328 of 342)	95.0 % (458 of 482)	96.2 % (154 of 160)
Sidechain	90.0 % (1037 of 1152)	89.6 % (632 of 705)	90.3 % (389 of 431)	100.0 % (16 of 16)
Aromatic	74.2 % (135 of 182)	74.7 % (68 of 91)	72.7 % (64 of 88)	100.0 % (3 of 3)
Methyl	97.2 % (140 of 144)	97.2 % (70 of 72)	97.2 % (70 of 72)

1. MvR76

MIKRGFPAVL DENTEILILG SLPSDESIRK QQYYGNPGND FWRLVGHAIG ENLQDMAYEK KLKTLKHNRI GLWDVFKAGS REGSQDSKIG DEEINDFSGL KEMVPKLRLI CFNGRKAGEY EPLLRGMGYE TKVLPSSSGA NRRFSKNRES EWEAVFRHLE HHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-5% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	MvR76	[U-5% 13C; U-100% 15N]	1.15 mM
11	MES	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	calcium chloride	natural abundance	5 mM
14	DTT	natural abundance	10 mM
15	sodium azide	natural abundance	0.02 %
16	DSS	natural abundance	50 uM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N MvR76 aligned in 4% C12E5 PEG

#	Name	Isotope labeling	Concentration
19	MvR76	[U-5% 13C; U-100% 15N]	0.80 mM
20	MES	natural abundance	20 mM
21	sodium chloride	natural abundance	100 mM
22	calcium chloride	natural abundance	5 mM
23	DTT	natural abundance	10 mM
24	sodium azide	natural abundance	0.02 %
25	DSS	natural abundance	50 uM
26	C12E5 PEG	natural abundance	4 %
27	H2O	natural abundance	90 %
28	D2O	natural abundance	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N MvR76 aligned in 7% PAG

#	Name	Isotope labeling	Concentration
29	MvR76	[U-5% 13C; U-100% 15N]	1.15 mM
30	MES	natural abundance	20 mM
31	sodium chloride	natural abundance	100 mM
32	calcium chloride	natural abundance	5 mM
33	DTT	natural abundance	10 mM
34	sodium azide	natural abundance	0.02 %
35	DSS	natural abundance	50 uM
36	Polyacrylamide gel	natural abundance	7 %
37	H2O	natural abundance	90 %
38	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2L3F, Strand ID: A Detail

RDC

210 RDC values in 2 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail

Release date

2011-10-09

Related entities 1. MvR76, : 1 : 3 entities Detail

Experiments performed 22 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

3 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

4 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

5 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

6 2D 1H-13C HSQC high resolution (L/V methyl stereoassignment)

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-5% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	MvR76	[U-5% 13C; U-100% 15N]	1.15 mM
11	MES	natural abundance	20 mM
12	sodium chloride	natural abundance	100 mM
13	calcium chloride	natural abundance	5 mM
14	DTT	natural abundance	10 mM
15	sodium azide	natural abundance	0.02 %
16	DSS	natural abundance	50 uM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

7 3D HNCO

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

8 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

9 3D HNCA

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

10 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

11 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

12 3D HNCACB

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

13 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

14 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

15 3D CCH-TOCSY

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

16 3D HCCH-COSY

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

17 3D HNHA

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

18 3D HBHANH

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

19 1D 15N T1 and T2

Instrument

Bruker Avance - 600 MHz 1.7-mm TCI microcryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

20 2D 1H-15N hetNOE

Instrument

Bruker Avance - 800 MHz 5-mm TXI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.94 mM [U-100% 13C; U-100% 15N] MvR76, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	MvR76	[U-100% 13C; U-100% 15N]	0.94 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	50 uM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

21 NH J-modulation

Instrument

Varian INOVA - 600 MHz 5-mm HCN coldprobe

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N MvR76 aligned in 4% C12E5 PEG

#	Name	Isotope labeling	Concentration
19	MvR76	[U-5% 13C; U-100% 15N]	0.80 mM
20	MES	natural abundance	20 mM
21	sodium chloride	natural abundance	100 mM
22	calcium chloride	natural abundance	5 mM
23	DTT	natural abundance	10 mM
24	sodium azide	natural abundance	0.02 %
25	DSS	natural abundance	50 uM
26	C12E5 PEG	natural abundance	4 %
27	H2O	natural abundance	90 %
28	D2O	natural abundance	10 %

22 NH J-modulation

Instrument

Varian INOVA - 600 MHz 5-mm HCN coldprobe

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 15N MvR76 aligned in 7% PAG

#	Name	Isotope labeling	Concentration
29	MvR76	[U-5% 13C; U-100% 15N]	1.15 mM
30	MES	natural abundance	20 mM
31	sodium chloride	natural abundance	100 mM
32	calcium chloride	natural abundance	5 mM
33	DTT	natural abundance	10 mM
34	sodium azide	natural abundance	0.02 %
35	DSS	natural abundance	50 uM
36	Polyacrylamide gel	natural abundance	7 %
37	H2O	natural abundance	90 %
38	D2O	natural abundance	10 %

nullKeywords NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-2, Protein NMR, Protein Structure Initiative, Structural Genomics, Target MvR76

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Found 1 Document (4.885 seconds)

BMRB: 17191

Sample #1

Sample #2

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Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MvR76, : 1 : 3 entities Detail

Experiments performed 22 experiments Detail

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